Scientific reports, 2019-05, Vol.9 (1), p.6978, Article 6978
2019
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- Autor(en) / Beteiligte
- Titel
- Tobacco-Related Alterations in Airway Gene Expression are Rapidly Reversed Within Weeks Following Smoking-Cessation
- Ist Teil von
- Scientific reports, 2019-05, Vol.9 (1), p.6978, Article 6978
- Ort / Verlag
- England: Nature Publishing Group
- Erscheinungsjahr
- 2019
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- The physiologic response to tobacco smoke can be measured by gene-expression profiling of the airway epithelium. Temporal resolution of kinetics of gene-expression alterations upon smoking-cessation might delineate distinct biological processes that are activated during recovery from tobacco smoke exposure. Using whole genome gene-expression profiling of individuals initiating a smoking-cessation attempt, we sought to characterize the kinetics of gene-expression alterations in response to short-term smoking-cessation in the nasal epithelium. RNA was extracted from the nasal epithelial of active smokers at baseline and at 4, 8, 16, and 24-weeks after smoking-cessation and put onto Gene ST arrays. Gene-expression levels of 119 genes were associated with smoking-cessation (FDR < 0.05, FC ≥1.7) with a majority of the changes occurring by 8-weeks and a subset changing by 4-weeks. Genes down-regulated by 4- and 8-weeks post-smoking-cessation were involved in xenobiotic metabolism and anti-apoptotic functions respectively. These genes were enriched among genes previously found to be induced in smokers and following short-term in vitro exposure of airway epithelial cells to cigarette smoke (FDR < 0.05). Our findings suggest that the nasal epithelium can serve as a minimally-invasive tool to measure the reversible impact of smoking and broadly, may serve to assess the physiological impact of changes in smoking behavior.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2045-2322eISSN: 2045-2322DOI: 10.1038/s41598-019-43295-3Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6502805
- Format
- –
- Schlagworte
- Adult, Apoptosis, Boston - epidemiology, Cigarette smoke, Epithelial cells, Epithelium, Female, Gene expression, Gene Expression Profiling, Gene Expression Regulation - drug effects, Genetic Markers, Genomes, Humans, Male, Middle Aged, Nasal Mucosa - drug effects, Nasal Mucosa - metabolism, Recovery of Function - genetics, Respiratory tract, Ribonucleic acid, RNA, Smoking, Smoking Cessation - statistics & numerical data, Tobacco, Tobacco smoke, Tobacco Smoking - adverse effects, Tobacco Smoking - epidemiology, Tobacco Smoking - genetics