RNA (Cambridge), 2019-05, Vol.25 (5), p.600-606
2019
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Details
- Autor(en) / Beteiligte
- Titel
- High-resolution crystal structures of ribosome-bound chloramphenicol and erythromycin provide the ultimate basis for their competition
- Ist Teil von
- RNA (Cambridge), 2019-05, Vol.25 (5), p.600-606
- Ort / Verlag
- United States: Cold Spring Harbor Laboratory Press
- Erscheinungsjahr
- 2019
- Quelle
- Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals
- Beschreibungen/Notizen
- The 70S ribosome is a major target for antibacterial drugs. Two of the classical antibiotics, chloramphenicol (CHL) and erythromycin (ERY), competitively bind to adjacent but separate sites on the bacterial ribosome: the catalytic peptidyl transferase center (PTC) and the nascent polypeptide exit tunnel (NPET), respectively. The previously reported competitive binding of CHL and ERY might be due either to a direct collision of the two drugs on the ribosome or due to a drug-induced allosteric effect. Because of the resolution limitations, the available structures of these antibiotics in complex with bacterial ribosomes do not allow us to discriminate between these two possible mechanisms. In this work, we have obtained two crystal structures of CHL and ERY in complex with the 70S ribosome at a higher resolution (2.65 and 2.89 Å, respectively) allowing unambiguous placement of the drugs in the electron density maps. Our structures provide evidence of the direct collision of CHL and ERY on the ribosome, which rationalizes the observed competition between the two drugs.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1355-8382eISSN: 1469-9001DOI: 10.1261/rna.069260.118Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6467010
- Format
- –
- Schlagworte
- 70S ribosome, Allosteric properties, Anti-Bacterial Agents - chemistry, Anti-Bacterial Agents - pharmacology, antibiotic, Antibiotics, BASIC BIOLOGICAL SCIENCES, Binding Sites, Binding, Competitive, Chloramphenicol, Chloramphenicol - chemistry, Chloramphenicol - pharmacology, Chlorophyll, Competition, Crystallography, X-Ray, Drugs, Erythromycin, Erythromycin - chemistry, Erythromycin - pharmacology, Escherichia coli - chemistry, Escherichia coli - drug effects, Escherichia coli - genetics, Escherichia coli - metabolism, inhibition of translation, Models, Molecular, nascent peptide exit tunnel, peptidyl transferase center, Peptidyl Transferases - antagonists & inhibitors, Peptidyl Transferases - chemistry, Peptidyl Transferases - genetics, Peptidyl Transferases - metabolism, Protein Binding, Protein Biosynthesis, Protein Conformation, Ribosome Subunits - drug effects, Ribosome Subunits - genetics, Ribosome Subunits - metabolism, Ribosome Subunits - ultrastructure, Ribosomes, Thermus thermophilus - chemistry, Thermus thermophilus - drug effects, Thermus thermophilus - genetics, Thermus thermophilus - metabolism, X-ray structure