Scientific reports, 2019-04, Vol.9 (1), p.5610-5610, Article 5610
2019
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- Autor(en) / Beteiligte
- Titel
- Alterations of protein expression of phospholamban, ZASP and plakoglobin in human atria in subgroups of seniors
- Ist Teil von
- Scientific reports, 2019-04, Vol.9 (1), p.5610-5610, Article 5610
- Ort / Verlag
- England: Nature Publishing Group
- Erscheinungsjahr
- 2019
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- The mature mammalian myocardium contains composite junctions (areae compositae) that comprise proteins of adherens junctions as well as desmosomes. Mutations or deficiency of many of these proteins are linked to heart failure and/or arrhythmogenic cardiomyopathy in patients. We firstly wanted to address the question whether the expression of these proteins shows an age-dependent alteration in the atrium of the human heart. Right atrial biopsies, obtained from patients undergoing routine bypass surgery for coronary heart disease were subjected to immunohistology and/or western blotting for the plaque proteins plakoglobin (γ-catenin) and plakophilin 2. Moreover, the Z-band protein cypher 1 (Cypher/ZASP) and calcium handling proteins of the sarcoplasmic reticulum (SR) like phospholamban, SERCA and calsequestrin were analyzed. We noted expression of plakoglobin, plakophilin 2 and Cypher/ZASP in these atrial preparations on western blotting and/or immunohistochemistry. There was an increase of Cypher/ZASP expression with age. The present data extend our knowledge on the expression of anchoring proteins and SR regulatory proteins in the atrium of the human heart and indicate an age-dependent variation in protein expression. It is tempting to speculate that increased expression of Cypher/ZASP may contribute to mechanical changes in the aging human myocardium.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2045-2322eISSN: 2045-2322DOI: 10.1038/s41598-019-42141-wTitel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6449388
- Format
- –
- Schlagworte
- Adaptor Proteins, Signal Transducing - genetics, Adaptor Proteins, Signal Transducing - metabolism, Adherens junctions, Age Factors, Aged, Aged, 80 and over, Aging, Arrhythmias, Cardiac - metabolism, Atrium, Blotting, Western, Ca2+-transporting ATPase, Calcium, Calcium-Binding Proteins - genetics, Calcium-Binding Proteins - metabolism, Calsequestrin, Cardiomyopathies - pathology, Cardiomyopathy, Cardiomyopathy, Dilated - genetics, Cardiomyopathy, Dilated - physiopathology, Cardiovascular disease, Catenin, Congestive heart failure, Coronary artery disease, Desmosomes, Female, gamma Catenin - genetics, gamma Catenin - metabolism, Heart, Heart Atria - metabolism, Heart Atria - pathology, Heart Failure - metabolism, Humans, Immunohistochemistry, LIM Domain Proteins - genetics, LIM Domain Proteins - metabolism, Male, Middle Aged, Myocardium, Myocardium - metabolism, Phospholamban, Protein expression, Proteins, Regulatory proteins, Sarcomeres - metabolism, Sarcoplasmic reticulum, Sarcoplasmic Reticulum - metabolism, Surgery, Transcriptome - genetics, Western blotting