Details

Autor(en) / Beteiligte

• Wang, Yuli
• Gunasekara, Dulan B
• Attayek, Peter J
• Reed, Mark I
• DiSalvo, Matthew
• Nguyen, Daniel L
• Dutton, Johanna S
• Lebhar, Michael S
• Bultman, Scott J
• Sims, Christopher E
• Magness, Scott T
• Allbritton, Nancy L

Titel

In Vitro Generation of Mouse Colon Crypts

Ist Teil von

• ACS biomaterials science & engineering, 2017-10, Vol.3 (10), p.2502-2513

Ort / Verlag

United States: American Chemical Society

Erscheinungsjahr

2017

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Organoid culture has had a significant impact on in vitro studies of the intestinal epithelium; however, the exquisite architecture, luminal accessibility, and lineage compartmentalization found in vivo has not been recapitulated in the organoid systems. We have used a microengineered platform with suitable extracellular matrix contacts and stiffness to generate a self-renewing mouse colonic epithelium that replicates key architectural and physiological functions found in vivo, including a surface lined with polarized crypts. Chemical gradients applied to the basal–luminal axis compartmentalized the stem/progenitor cells and promoted appropriate lineage differentiation along the in vitro crypt axis so that the tissue possessed a crypt stem cell niche as well as a layer of differentiated cells covering the luminal surface. This new approach combining microengineered scaffolds, native chemical gradients, and biophysical cues to control primary epithelium ex vivo can serve as a highly functional and physiologically relevant in vitro tissue model.