Journal of controlled release, 2019-02, Vol.295, p.21-30
2019
Details
- Autor(en) / Beteiligte
- Titel
- Microfluidic assembly of hydrogel-based immunogenic tumor spheroids for evaluation of anticancer therapies and biomarker release
- Ist Teil von
- Journal of controlled release, 2019-02, Vol.295, p.21-30
- Ort / Verlag
- Netherlands: Elsevier B.V
- Erscheinungsjahr
- 2019
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Diffuse large B cell lymphoma (DLBCL), the most common subtype of Non-Hodgkin lymphoma, exhibits pathologic heterogeneity and a dynamic immunogenic tumor microenvironment (TME). However, the lack of preclinical in vitro models of DLBCL TME hinders optimal therapeutic screening. This study describes the development of an integrated droplet microfluidics-based platform for high-throughput generation of immunogenic DLBCL spheroids. The spheroids consist of three cell types (cancer, fibroblast and lymphocytes) in a novel hydrogel combination of alginate and puramatrix, which promoted cell adhesion and aggregation. This system facilitates dynamic analysis of cellular interaction, proliferation and therapeutic efficacy via spatiotemporal monitoring and secretome profiling. The immunomodulatory drug lenalidomide had direct anti-proliferative effect on activated B-cell like DLBCL spheroids and reduced several cytokines and other markers (e.g., CCL2, CCL3, CCL4, CD137 and ANG-1 levels) compared with untreated spheroids. Collectively, this novel spheroid platform will enable high-throughput anti-cancer therapeutic screening in a semi-automated manner. [Display omitted]
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0168-3659eISSN: 1873-4995DOI: 10.1016/j.jconrel.2018.12.010Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6396303
- Format
- –
- Schlagworte
- 3D culture, Alginate, Alginates - chemistry, Antineoplastic Agents - pharmacology, Biomarkers, Tumor - analysis, Biomarkers, Tumor - immunology, Cell Culture Techniques - instrumentation, Cell Culture Techniques - methods, Cell Line, Cell Proliferation - drug effects, Cell Survival - drug effects, Cells, Cultured, Coculture Techniques - instrumentation, Coculture Techniques - methods, Drug Screening Assays, Antitumor - instrumentation, Drug Screening Assays, Antitumor - methods, Equipment Design, Humans, Hydrogels - chemistry, Immunogenic spheroid, Immunologic Factors - pharmacology, Lab-On-A-Chip Devices, Lenalidomide - pharmacology, Lymphoma, Large B-Cell, Diffuse - drug therapy, Lymphoma, Large B-Cell, Diffuse - immunology, Microfluidics, Puramatrix, Spheroids, Cellular - drug effects, Spheroids, Cellular - immunology, Tumor Cells, Cultured - drug effects, Tumor Cells, Cultured - immunology