Bioscience reports, 2019-01, Vol.39 (1)
2019
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- Autor(en) / Beteiligte
- Titel
- LDH-A promotes malignant behavior via activation of epithelial-to-mesenchymal transition in lung adenocarcinoma
- Ist Teil von
- Bioscience reports, 2019-01, Vol.39 (1)
- Ort / Verlag
- England: Portland Press Ltd
- Erscheinungsjahr
- 2019
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Lactate dehydrogenase A (LDH-A) is a key enzyme during glycolysis, which increases the synthesis of related proteins and has elevated activity in cancer cells. The role of LDH-A in lung adenocarcinoma (LUAD) progression was investigated in the present study. Expression levels of LDH-A were assessed in LUAD samples, and the relationship between LDH-A expression status and the prognosis of LUAD patients was confirmed. The effect of LDH-A on proliferation, invasion, migration, and colony formation of cancer cells was assessed. We further determined the role of LDH-A in tumor growth by using xenograft LUAD tumor models. The potential mechanism of LDH-A promotion in LUAD progression was explored. LDH-A showed an abnormally high expression in LUAD, which is closely associated with poor prognosis in patients with LUAD. In experiments, silencing LDH-A expression in LUAD cells could effectively inhibit proliferation, invasion, migration, and colony formation of cancer cells. In experiments, tumor growth was markedly inhibited by LDH-A silencing in a xenograft model of LUAD. Notably, LDH-A could also promote tumor progression by regulating epithelial-mesenchymal transition (EMT)-related molecules. LDH-A can promote the malignant biological behaviors of LUAD cells, and thus can be a potential target for LUAD treatment.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0144-8463eISSN: 1573-4935DOI: 10.1042/bsr20181476Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6331674
- Format
- –
- Schlagworte
- A549 Cells, Adenocarcinoma of Lung - genetics, Adenocarcinoma of Lung - mortality, Adenocarcinoma of Lung - pathology, Adenocarcinoma of Lung - therapy, Adult, Aged, Animals, Antigens, CD - genetics, Antigens, CD - metabolism, Cadherins - genetics, Cadherins - metabolism, Cell Line, Tumor, Cell Movement, Cell Proliferation, Epithelial-Mesenchymal Transition - genetics, Female, Gene Expression Regulation, Neoplastic, Humans, L-Lactate Dehydrogenase - antagonists & inhibitors, L-Lactate Dehydrogenase - genetics, L-Lactate Dehydrogenase - metabolism, Lung Neoplasms - genetics, Lung Neoplasms - mortality, Lung Neoplasms - pathology, Lung Neoplasms - therapy, Lymphatic Metastasis, Male, Mice, Nude, Middle Aged, Prognosis, RNA, Small Interfering - genetics, RNA, Small Interfering - metabolism, Signal Transduction, Survival Analysis, Vimentin - genetics, Vimentin - metabolism, Xenograft Model Antitumor Assays, Zinc Finger E-box-Binding Homeobox 1 - genetics, Zinc Finger E-box-Binding Homeobox 1 - metabolism