Details

Autor(en) / Beteiligte

• Weng, Li
• Ma, Jun
• Jia, Yi-Ping
• Wu, Shao-Qiu
• Liu, Bin-Yan
• Cao, Yan
• Yin, Xiang
• Shang, Ming-Yi
• Mao, Ai-Wu

Titel

MiR-4262 promotes cell apoptosis and inhibits proliferation of colon cancer cells: involvement of GALNT4

Ist Teil von

• American journal of translational research, 2018-01, Vol.10 (12), p.3969-3977

Ort / Verlag

United States: e-Century Publishing Corporation

Erscheinungsjahr

2018

Quelle

EZB*

Beschreibungen/Notizen

• A role of microRNA-4262 (miR-4262) in the carcinogenesis of colon cancer remains undetermined. In this study, we studied the effects and mechanisms of miR-4262 to the colon cancer cell proliferation and apoptosis. We found that the levels of miR-4262 significantly down-regulated in colon cancer tissue, compared to the paired adjacent non-tumor colon tissue. The miR-4262 levels in colon cancer cell lines were significantly lower than those in control normal colon tissues. Transfection with the miR-4262 mimic decreased the cell proliferation and increased cell apoptosis in colon cancer cells, while transfection with the antisense of miR-4262 (as-miR-4262) increased cell proliferation and suppressed cell apoptosis in colon cancer cells. Bioinformatics analyses showed that GALNT4 was a potential target gene of miR-4262. The luciferase activities assay and Western blot verified that miR-4262 targeted GALNT4 mRNA to modulate its protein levels. When we treated cells with miR-4262 and GALN4 siRNA, the cell viability was significantly decreased. Together, our study suggests that aberrantly expressed miR-4262 may affect cell apoptosis and proliferation of human colon cancer cells via GALNT4, which appears to be a promising therapeutic target for colon cancer.