Details

Autor(en) / Beteiligte

• Yu, H.T.
• Ding, X. L.
• Li, N.
• Zhang, X. Y.
• Zeng, X. F.
• Wang, S.
• Liu, H. B.
• Wang, Y. M.
• Jia, H. M.
• Qiao, S. Y.

Titel

Dietary supplemented antimicrobial peptide microcin J25 improves the growth performance, apparent total tract digestibility, fecal microbiota, and intestinal barrier function of weaned pigs1,2

Ist Teil von

• Journal of animal science, 2017-11, Vol.95 (11), p.5064-5076

Ort / Verlag

Oxford University Press

Erscheinungsjahr

2017

Quelle

EZB Free E-Journals

Beschreibungen/Notizen

• Abstract Microcin J25 (MccJ25) is an antimicrobial peptide produced by a fecal strain of Escherichia coli containing 21 AA. This study was performed primarily to evaluate the effects of MccJ25 as a potential substitute for antibiotics (AB) on growth performance, nutrient digestibility, fecal microbiota, and intestinal barrier function in weaned pigs. In the present study, 180 weaned pigs (7.98 ± 0.29 kg initial BW) were randomly assigned to 1 of 5 treatments, including a basal diet (CON) and CON supplemented with AB (20 mg/kg colistin sulfate; ABD) or 0.5, 1.0, and 2.0 mg/kg MccJ25. On d 0 to 14, dietary supplementation with MccJ25 and ABD had positive effects on ADG, ADFI, diarrhea incidence, and G:F (P < 0.05). Pigs fed the 2.0 mg/kg MccJ25 diet had greater ADG (P < 0.05) and marginally greater G:F (P < 0.10) compared with pigs fed the ABD diet. Compared with the CON diet, the 2.0 mg/kg MccJ25 diet sharply improved (P < 0.05) ADG and G:F and decreased (P < 0.05) diarrhea incidence (d 15 to 28 and d 0 to 28). Apparent digestibility of nutrients in pigs fed 1.0 and 2.0 mg/kg MccJ25 was improved (P < 0.05) compared with that of pigs fed CON and ABD. The serum cytokines IL-6 and IL-1β and tumor necrosis factor-α levels in pigs fed MccJ25 were greater than in pigs fed CON (P < 0.05). Additionally, the IL-10 concentration in pigs fed MccJ25 was sharply increased (P < 0.05) compared with that of pigs fed CON. Pigs fed 1.0 and 2.0 mg/kg MccJ25 diets had remarkably decreased D-lactate, diamine oxidase, and endotoxin concentrations and fecal E. coli numbers (P < 0.05) and improved fecal Lactobacillus and Bifidobacterium numbers (P < 0.05). Compared with the ABD diet, the diet containing 2.0 mg/kg MccJ25 did not increase D-lactate, diamine oxidase, and endotoxin (d 14) concentrations (P < 0.05) or decrease the Lactobacillus and Bifidobacterium (d 28) numbers (P < 0.05). The diets containing 1.0 and 2.0 mg/kg MccJ25 and ABD (d 28) improved lactate concentration and short-chain fatty acid concentrations, including acetate, propionate, and butyrate, in feces (P < 0.05). Moreover, the pigs fed 2.0 mg/kg MccJ25 had greater lactate, butyrate (d 14), and propionate concentrations than the pigs fed the ABD diet (P < 0.05). In conclusion, dietary supplemented MccJ25 effectively improved performance, attenuated diarrhea and systematic inflammation, enhanced intestinal barrier function, and improved fecal microbiota composition of weaned pigs. Therefore, MccJ25 could be a potential effective alternative to AB for weaned pigs.