Details

Autor(en) / Beteiligte

• Chang, Yan-Yan
• Gong, Xin-Wei
• Gong, Hai-Qing
• Liang, Pei-Ji
• Zhang, Pu-Ming
• Lu, Qin-Chi

Titel

GABAA Receptor Activity Suppresses the Transition from Inter-ictal to Ictal Epileptiform Discharges in Juvenile Mouse Hippocampus

Ist Teil von

• Neuroscience bulletin, 2018-12, Vol.34 (6), p.1007-1016

Ort / Verlag

Singapore: Springer Singapore

Erscheinungsjahr

2018

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Exploring the transition from inter-ictal to ictal epileptiform discharges (IDs) and how GABA A receptor-mediated action affects the onset of IDs will enrich our understanding of epileptogenesis and epilepsy treatment. We used Mg 2+ -free artificial cerebrospinal fluid (ACSF) to induce epileptiform discharges in juvenile mouse hippocampal slices and used a micro-electrode array to record the discharges. After the slices were exposed to Mg 2+ -free ACSF for 10 min–20 min, synchronous recurrent seizure-like events were recorded across the slices, and each event evolved from inter-ictal epileptiform discharges (IIDs) to pre-ictal epileptiform discharges (PIDs), and then to IDs. During the transition from IIDs to PIDs, the duration of discharges increased and the inter-discharge interval decreased. After adding 3 μmol/L of the GABA A receptor agonist muscimol, PIDs and IDs disappeared, and IIDs remained. Further, the application of 10 μmol/L muscimol abolished all the epileptiform discharges. When the GABA A receptor antagonist bicuculline was applied at 10 μmol/L, IIDs and PIDs disappeared, and IDs remained at decreased intervals. These results indicated that there are dynamic changes in the hippocampal network preceding the onset of IDs, and GABA A receptor activity suppresses the transition from IIDs to IDs in juvenile mouse hippocampus.