Neuroscience letters, 2018-11, Vol.687, p.31-36
2018
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- Autor(en) / Beteiligte
- Titel
- Proteasome limits plasticity-related signaling to the nucleus in the hippocampus
- Ist Teil von
- Neuroscience letters, 2018-11, Vol.687, p.31-36
- Ort / Verlag
- Ireland: Elsevier B.V
- Erscheinungsjahr
- 2018
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- •We studied proteasomal regulation of nuclear signaling in synaptic plasticity.•We used subthreshold long-term potentiation as our experimental model.•We examined phosphorylation of cAMP-responsive element binding protein (CREB).•Inhibition of the proteasome increased phosphorylated CREB (pCREB) in the nucleus.•Blocking plasticity-related protein kinases prevented increase in pCREB. Proteolysis by the ubiquitin-proteasome pathway has pleiotropic effects on both induction and maintenance of long-term synaptic plasticity. In this study, we examined the effect of proteasome inhibition on signaling to the nucleus during late-phase long-term potentiation. When a subthreshold L-LTP induction protocol was used, proteasome inhibition led to a significant increase in phosphorylated CREB (pCREB) in the nucleus. Inhibitors of cAMP-dependent protein kinase/protein kinase A, extracellular signal-regulated kinase and cGMP-dependent protein kinase/protein kinase G all blocked the proteasome-inhibition-mediated increase in nuclear pCREB after subthreshold stimulation. These results lay the groundwork for understanding a novel role for the proteasome in limiting signaling to the nucleus in the absence of adequate synaptic stimulation.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0304-3940eISSN: 1872-7972DOI: 10.1016/j.neulet.2018.09.017Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6231982
- Format
- –
- Schlagworte
- Animals, Cell Nucleus - drug effects, Cell Nucleus - metabolism, Cyclic AMP Response Element-Binding Protein - metabolism, Gene expression, Hippocampus - drug effects, Hippocampus - metabolism, Long-term potentiation, Long-Term Potentiation - drug effects, Long-Term Potentiation - physiology, Male, Mice, Mice, Inbred C57BL, Neuronal Plasticity - drug effects, Neuronal Plasticity - physiology, Organ Culture Techniques, Proteasome Endopeptidase Complex - metabolism, Proteasome Inhibitors - pharmacology, Protein degradation, Protein kinase, Signal Transduction - drug effects, Signal Transduction - physiology, Transcription factor