Genetics (Austin), 2018-10, Vol.210 (2), p.607-619
2018
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- Autor(en) / Beteiligte
- Titel
- Sucrose Signaling Regulates Anthocyanin Biosynthesis Through a MAPK Cascade in Arabidopsis thaliana
- Ist Teil von
- Genetics (Austin), 2018-10, Vol.210 (2), p.607-619
- Ort / Verlag
- United States: Genetics Society of America
- Erscheinungsjahr
- 2018
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- Anthocyanin accumulation specifically depends on sucrose (Suc) signaling. However, the molecular basis of this process remains unknown. In this study, pull-down assays identified ETHYLENE-INSENSITIVE3 (EIN3), a component of both sugar signaling or/and metabolism. This protein interacted with YDA, and the physiological relevance of this interaction was confirmed by co-immunoprecipitation, yeast two-hybrid (Y2H) assay, and bimolecular fluorescence complementation. ( ) double-mutant seedlings, but not seedlings, showed anthocyanin accumulation. Furthermore, suppressed anthocyanin accumulation in plants. Thus, may form a sugar-mediated gene cascade integral to the regulation of anthocyanin accumulation. Moreover, the gene cascade module directly targeted the promoter of ( ) by direct EIN3 binding. Collectively, our data inferred a molecular model where the signaling cascade of the YDA-EIN3-TT8 appeared to target via EIN3, thereby modulating Suc signaling-mediated anthocyanin accumulation.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1943-2631, 0016-6731eISSN: 1943-2631DOI: 10.1534/genetics.118.301470Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6216600
- Format
- –
- Schlagworte
- Accumulation, Anthocyanins - biosynthesis, Anthocyanins - genetics, Arabidopsis - genetics, Arabidopsis Proteins - genetics, Arabidopsis Proteins - metabolism, Biosynthesis, Complementation, Ethylene, Flavonoids, Flowers & plants, Fluorescence, Gene expression, Genetics, Immunoprecipitation, Investigations, Kinases, MAP kinase, MAP Kinase Signaling System, Metabolism, Nuclear Proteins - genetics, Nuclear Proteins - metabolism, Physiology, Protein Binding, Protein turnover, Proteins, Seedlings, Signaling, Sucrose, Sucrose - metabolism, Sugar, Transcription factors, Transcription Factors - genetics, Transcription Factors - metabolism, Yeast