Details

Autor(en) / Beteiligte

• Kovar, Lynsey
• Nageswara-Rao, Madhugiri
• Ortega-Rodriguez, Sealtiel
• Dugas, Diana V
• Straub, Shannon
• Cronn, Richard
• Strickler, Susan R
• Hughes, Colin E
• Hanley, Kathryn A
• Rodriguez, Deyra N
• Langhorst, Bradley W
• Dimalanta, Eileen T
• Bailey, C Donovan
• Lavrov, Dennis

Titel

PacBio-Based Mitochondrial Genome Assembly of Leucaena trichandra (Leguminosae) and an Intrageneric Assessment of Mitochondrial RNA Editing

Ist Teil von

• Genome biology and evolution, 2018-09, Vol.10 (9), p.2501-2517

Ort / Verlag

England: Oxford University Press

Erscheinungsjahr

2018

Quelle

MEDLINE

Beschreibungen/Notizen

• Reconstructions of vascular plant mitochondrial genomes (mt-genomes) are notoriously complicated by rampant recombination that has resulted in comparatively few plant mt-genomes being available. The dearth of plant mitochondrial resources has limited our understanding of mt-genome structural diversity, complex patterns of RNA editing, and the origins of novel mt-genome elements. Here, we use an efficient long read (PacBio) iterative assembly pipeline to generate mt-genome assemblies for Leucaena trichandra (Leguminosae: Caesalpinioideae: mimosoid clade), providing the first assessment of non-papilionoid legume mt-genome content and structure to date. The efficiency of the assembly approach facilitated the exploration of alternative structures that are common place among plant mitochondrial genomes. A compact version (729 kbp) of the recovered assemblies was used to investigate sources of mt-genome size variation among legumes and mt-genome sequence similarity to the legume associated root holoparasite Lophophytum. The genome and an associated suite of transcriptome data from select species of Leucaena permitted an in-depth exploration of RNA editing in a diverse clade of closely related species that includes hybrid lineages. RNA editing in the allotetraploid, Leucaena leucocephala, is consistent with co-option of nearly equal maternal and paternal C-to-U edit components, generating novel combinations of RNA edited sites. A preliminary investigation of L. leucocephala C-to-U edit frequencies identified the potential for a hybrid to generate unique pools of alleles from parental variation through edit frequencies shared with one parental lineage, those intermediate between parents, and transgressive patterns.