Details

Autor(en) / Beteiligte

• Yudasaka, Masako
• Yomogida, Yohei
• Zhang, Minfang
• Nakahara, Masako
• Kobayashi, Norihiko
• Tanaka, Takeshi
• Okamatsu-Ogura, Yuko
• Saeki, Kumiko
• Kataura, Hiromichi

Titel

Fasting-dependent Vascular Permeability Enhancement in Brown Adipose Tissues Evidenced by Using Carbon Nanotubes as Fluorescent Probes

Ist Teil von

• Scientific reports, 2018-09, Vol.8 (1), p.14446-13, Article 14446

Ort / Verlag

England: Nature Publishing Group

Erscheinungsjahr

2018

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• Brown adipose tissue (BAT), which is composed of thermogenic brown adipocytes (BA) and non-parenchymal components including vasculatures and extracellular matrix, contribute to the maintenance of body temperature. BAT distribution is detected by positron emission tomography-computed tomography (PET/CT) using F-fluorodeoxy glucose ( F-FDG) or single-photon-emission computed tomography-computed tomography (SPECT/CT) using [ / I]-beta-methyl-p-iodophenyl-pentadecanoic acid. Although sympathetic nerve activity and thermogenic capacity of BA is downregulated under fasting conditions in mice, fasting-dependent structural changes and fluid kinetics of BAT remain unknown. Here we show that the fasting induces fine and reversible structural changes in the non-parenchymal region in murine BAT with widened intercellular spaces and deformed collagen bands as revealed by electron microscopy. Interestingly, a newly introduced near infrared fluorescent probe of single-walled carbon nanotubes (CNTs) coated with phospholipid polyethylene glycol (PLPEG) easily demonstrated enhanced vascular permeability in BAT by the fasting. PLPEG-CNTs extravasated and remained in intercellular spaces or further redistributed in parenchymal cells in fasted mice, which is a previously unknown phenomenon. Thus, PLPEG-CNTs provide a powerful tool to trace fluid kinetics in sub-tissue levels.