Details

Autor(en) / Beteiligte

• Silva-Neta, Helena L.
• Brelaz-de-Castro, Maria C. A.
• Chagas, Mardonny B. O.
• Mariz, Henrique A.
• Arruda, Rodrigo G. de
• Vasconcelos, Viviane F. de
• Pereira, Michelly C.
• Romano, Audrey
• Pitta, Ivan R.
• Marques, Claudia D. L.
• Duarte, Angela L. B. P.
• Rêgo, Moacyr J. B. M.
• Pitta, Maira G. R.
• Mahler, Michael
• Michael Mahler

Titel

CD4+CD45RA−FOXP3low Regulatory T Cells as Potential Biomarkers of Disease Activity in Systemic Lupus Erythematosus Brazilian Patients

Ist Teil von

• BioMed research international, 2018-01, Vol.2018, p.3419565-3419565

Ort / Verlag

New York: Hindawi

Erscheinungsjahr

2018

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Heren, we analyzed Treg cells as potential biomarkers of disease activity in systemic lupus erythematosus (SLE) patients. Peripheral blood mononuclear cells from 30 SLE patients (15 active: SLEDAI > 6/15 SLE remission: SLEDAI< 6) and 15 healthy volunteers were purified. Treg immunophenotyping was performed using CD4, CD25, CD45, CD127, and FOXP3 markers. CD4+FOXP3+ Treg activation state was investigated based on CD45RA and FOXP3 expression. To increase the accuracy of our findings, a multivariate linear regression was performed. We showed a significant increase in the frequency of CD4+FOXP3+ Treg cells in SLE patients. However, unlike all other Treg cells phenotypes analyzed, only eTreg (CD4+ F O X P 3 h i g h CD45RA-) (p=0.01) subtype was inversely correlated with disease activity while Foxp3+nontreg (CD4+ F O X P 3 l o w CD45RA-) (p=0.003) exerted a direct influence in the outcome of the disease. Foxp3+nontreg cells were the most consistent SLE active indicator, confirmed by multiple linear regression analyses. In summary, our results demonstrate Foxp3+nontreg cells as new biomarkers in the search of an effective therapeutic strategy in SLE.