Bioorganic & medicinal chemistry letters, 2016-02, Vol.26 (3), p.969-972
2016
Details
- Autor(en) / Beteiligte
- Titel
- Efficient enzymatic synthesis of l-rhamnulose and l-fuculose
- Ist Teil von
- Bioorganic & medicinal chemistry letters, 2016-02, Vol.26 (3), p.969-972
- Ort / Verlag
- England: Elsevier Ltd
- Erscheinungsjahr
- 2016
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- [Display omitted] l-Rhamnulose (6-deoxy-l-arabino-2-hexulose) and l-fuculose (6-deoxy-l-lyxo-2-hexulose) were prepared from l-rhamnose and l-fucose by a two-step strategy. In the first reaction step, isomerization of l-rhamnose to l-rhamnulose, or l-fucose to l-fuculose was combined with a targeted phosphorylation reaction catalyzed by l-rhamnulose kinase (RhaB). The by-products (ATP and ADP) were selectively removed by silver nitrate precipitation method. In the second step, the phosphate group was hydrolyzed to produce l-rhamnulose or l-fuculose with purity exceeding 99% in more than 80% yield (gram scale).
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0960-894XeISSN: 1464-3405DOI: 10.1016/j.bmcl.2015.12.051Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5984655
- Format
- –
- Schlagworte
- Biocatalysis, Chemical Precipitation, Chromatography, High Pressure Liquid, De-phosphorylation, Enzymatic synthesis, Fucose - metabolism, Hexoses - biosynthesis, Hexoses - chemistry, Hexoses - isolation & purification, l-Fuculose, l-Rhamnulose, Magnetic Resonance Spectroscopy, Phosphorylation, Phosphotransferases (Alcohol Group Acceptor) - metabolism, Rhamnose - analogs & derivatives, Rhamnose - biosynthesis, Rhamnose - chemistry, Rhamnose - isolation & purification, Rhamnose - metabolism, Silver Nitrate - chemistry