Details
- Autor(en) / Beteiligte
- Titel
- Cas13d Is a Compact RNA-Targeting Type VI CRISPR Effector Positively Modulated by a WYL-Domain-Containing Accessory Protein
- Ist Teil von
- Molecular cell, 2018-04, Vol.70 (2), p.327-339.e5
- Ort / Verlag
- United States: Elsevier Inc
- Erscheinungsjahr
- 2018
- Link zum Volltext
- Quelle
- Electronic Journals Library
- Beschreibungen/Notizen
- Bacterial class 2 CRISPR-Cas systems utilize a single RNA-guided protein effector to mitigate viral infection. We aggregated genomic data from multiple sources and constructed an expanded database of predicted class 2 CRISPR-Cas systems. A search for novel RNA-targeting systems identified subtype VI-D, encoding dual HEPN domain-containing Cas13d effectors and putative WYL-domain-containing accessory proteins (WYL1 and WYL-b1 through WYL-b5). The median size of Cas13d proteins is 190 to 300 aa smaller than that of Cas13a–Cas13c. Despite their small size, Cas13d orthologs from Eubacterium siraeum (Es) and Ruminococcus sp. (Rsp) are active in both CRISPR RNA processing and targeting, as well as collateral RNA cleavage, with no target-flanking sequence requirements. The RspWYL1 protein stimulates RNA cleavage by both EsCas13d and RspCas13d, demonstrating a common regulatory mechanism for divergent Cas13d orthologs. The small size, minimal targeting constraints, and modular regulation of Cas13d effectors further expands the CRISPR toolkit for RNA manipulation and detection. [Display omitted] •Type VI-D is a CRISPR-Cas system with a Cas13d effector and a WYL domain accessory•Cas13d is an RNA-guided RNase approximately 20% smaller than Cas13a–Cas13c effectors•WYL1 positively modulates Cas13d target and collateral RNase activity•Cas13d has minimal sequence and secondary structure requirements for targeting Compiling an expanded database of predicted class 2 CRISPR-Cas systems, Yan et al. identify and characterize subtype VI-D. Cas13d is an RNA-guided RNase effector with polyphyletic WYL-domain accessory proteins. One WYL1 ortholog enhances activity of divergent Cas13d orthologs. The small effector size and modular enhancement further expand RNA modification capabilities.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1097-2765eISSN: 1097-4164DOI: 10.1016/j.molcel.2018.02.028Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5935466
- Format
- –
- Schlagworte
- Amino Acid Sequence, Bacterial Proteins - chemistry, Bacterial Proteins - genetics, Bacterial Proteins - metabolism, Clustered Regularly Interspaced Short Palindromic Repeats, CRISPR, CRISPR-Associated Proteins - chemistry, CRISPR-Associated Proteins - genetics, CRISPR-Associated Proteins - metabolism, CRISPR-Cas, CRISPR-Cas Systems, Databases, Genetic, Escherichia coli - enzymology, Escherichia coli - genetics, Eubacterium - enzymology, Eubacterium - genetics, gene editing, Gene Editing - methods, Gene Expression Regulation, Bacterial, genome engineering, Nucleic Acid Conformation, Protein Domains, Protein Structure, Secondary, RNA editing, RNA Processing, Post-Transcriptional, RNA targeting, RNA, Bacterial - chemistry, RNA, Bacterial - genetics, RNA, Bacterial - metabolism, RNA, Guide, CRISPR-Cas Systems - genetics, RNA, Guide, CRISPR-Cas Systems - metabolism, Ruminococcus - enzymology, Ruminococcus - genetics, Structure-Activity Relationship, type VI CRISPR-Cas systems