Japanese Journal of Cancer Research, 1997-10, Vol.88 (10), p.953-958
1997
Details
- Autor(en) / Beteiligte
- Titel
- Expression of Interleukin‐6 and Its Effect on the Cell Growth of Gastric Carcinoma Cell Lines
- Ist Teil von
- Japanese Journal of Cancer Research, 1997-10, Vol.88 (10), p.953-958
- Ort / Verlag
- Oxford, UK: Blackwell Publishing Ltd
- Erscheinungsjahr
- 1997
- Link zum Volltext
- Quelle
- Wiley Online Library Journals Frontfile Complete
- Beschreibungen/Notizen
- The expression and the effect of IL‐6 were examined in human gastric carcinoma cell lines to determine whether IL‐6 serves as a growth stimulator. The expression of IL‐6 mRNA was detected in three (TMK‐1, MKN‐1, MKN‐7) of 8 gastric carcinoma cell lines. All three cell lines secreted IL‐6 into the culture fluid, in large amounts in the cases of MKN‐1 and MKN‐7 cells. Scatchard plot analysis of IL‐6 binding revealed that MKN‐1 and MKN‐7 cells had both high‐ and low‐affinity receptors. Cell growth of MKN‐1 and MKN‐7 cells was stimulated by IL‐6, while anti‐IL‐6 antibody inhibited growth. The expression of IL‐la mRNA by these three cell lines was induced by IL‐6. IL‐la increased the expression of mRNA for IL‐6 by TMK‐1 cells. These findings indicate that IL‐6 induced by IL‐la is an autocrine growth factor for some gastric carcinomas
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0910-5050eISSN: 1349-7006, 1876-4673DOI: 10.1111/j.1349-7006.1997.tb00314.xTitel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5921276
- Format
- –
- Schlagworte
- Adenocarcinoma - metabolism, Adenocarcinoma - pathology, Autocrine, Autocrine signalling, Biological and medical sciences, Blotting, Northern, Carcinoma, Carcinoma, Adenosquamous - metabolism, Carcinoma, Adenosquamous - pathology, Carcinoma, Signet Ring Cell - metabolism, Carcinoma, Signet Ring Cell - pathology, Cell culture, Cell Division - drug effects, Culture Media, Conditioned - chemistry, Cytokine, Cytokines, Gastric cancer, Gastric carcinoma cell line, Gastroenterology. Liver. Pancreas. Abdomen, Gene expression, Growth factors, Humans, IL‐6, Interleukin-1 - genetics, Interleukin-1 - metabolism, Interleukin-6 - genetics, Interleukin-6 - metabolism, Interleukin-6 - pharmacology, Medical sciences, mRNA, Receptors, Interleukin-6 - metabolism, RNA, Messenger - metabolism, Stomach Neoplasms - metabolism, Stomach Neoplasms - pathology, Stomach. Duodenum. Small intestine. Colon. Rectum. Anus, Tumor cell lines, Tumor Cells, Cultured, Tumors