Details

Autor(en) / Beteiligte

• Kalsi, Gurpreet
• Etzler, Marilynn E.

Titel

Localization of a Nod Factor-Binding Protein in Legume Roots and Factors Influencing Its Distribution and Expression1

Ist Teil von

• Plant physiology (Bethesda), 2000-11, Vol.124 (3), p.1039-1048

Ort / Verlag

American Society of Plant Physiologists

Erscheinungsjahr

2000

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• The roots of the legume Dolichos biflorus contain a lectin/nucleotide phosphohydrolase (Db-LNP) that binds to the Nod factor signals produced by rhizobia that nodulate this plant. In this study we show that Db-LNP is differentially distributed along the surface of the root axis in a pattern that correlates with the zone of nodulation of the root. Db-LNP is present on the surface of young and emerging root hairs and redistributes to the tips of the root hairs in response to treatment of the roots with a rhizobial symbiont or with a carbohydrate ligand. This redistribution does not occur in response to a non-symbiotic rhizobial strain or a root pathogen. Db-LNP is also present in the root pericycle where its level decreases upon initiation of nodule formation. Maximum levels of Db-LNP are found in 2-d-old roots, and the expression of this root protein is increased when the plants are grown in the absence of NO 3 − and NH 4 + . These results support the possibility that Db-LNP is involved in the initiation of the Rhizobium legume symbiosis.