Details

Autor(en) / Beteiligte

• Manzano, Aránzazu
• Herranz, Raúl
• den Toom, Leonardus A.
• te Slaa, Sjoerd
• Borst, Guus
• Visser, Martijn
• Medina, F. Javier
• van Loon, Jack J. W. A.

Titel

Novel, Moon and Mars, partial gravity simulation paradigms and their effects on the balance between cell growth and cell proliferation during early plant development

Ist Teil von

• NPJ microgravity, 2018-04, Vol.4 (1), p.9-11, Article 9

Ort / Verlag

London: Nature Publishing Group UK

Erscheinungsjahr

2018

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• Clinostats and Random Positioning Machine (RPM) are used to simulate microgravity, but, for space exploration, we need to know the response of living systems to fractional levels of gravity (partial gravity) as they exist on Moon and Mars. We have developed and compared two different paradigms to simulate partial gravity using the RPM, one by implementing a centrifuge on the RPM (RPM HW ), the other by applying specific software protocols to driving the RPM motors (RPM SW ). The effects of the simulated partial gravity were tested in plant root meristematic cells, a system with known response to real and simulated microgravity. Seeds of Arabidopsis thaliana were germinated under simulated Moon (0.17  g ) and Mars (0.38  g ) gravity. In parallel, seeds germinated under simulated microgravity (RPM), or at 1  g control conditions. Fixed root meristematic cells from 4-day grown seedlings were analyzed for cell proliferation rate and rate of ribosome biogenesis using morphometrical methods and molecular markers of the regulation of cell cycle and nucleolar activity. Cell proliferation appeared increased and cell growth was depleted under Moon gravity, compared with the 1  g control. The effects were even higher at the Moon level than at simulated microgravity, indicating that meristematic competence (balance between cell growth and proliferation) is also affected at this gravity level. However, the results at the simulated Mars level were close to the 1  g static control. This suggests that the threshold for sensing and responding to gravity alteration in the root would be at a level intermediate between Moon and Mars gravity. Both partial g simulation strategies seem valid and show similar results at Moon g -levels, but further research is needed, in spaceflight and simulation facilities, especially around and beyond Mars g levels to better understand more precisely the differences and constrains in the use of these facilities for the space biology community. Plant biology: partial gravity impacts root development Novel simulators of partial gravity show that the threshold for plants to sense and respond to gravity in their roots lies somewhere between gravity levels of the Moon and Mars. An international team led by Jack van Loon from the VU University Medical Center in Amsterdam, the Netherlands, reconfigured the hardware (include centrifugation) and software (new algorithm) on a microgravity-simulating instrument known as a Random Positioning Machine to produce fractional levels of gravity similar to those found on Mars (0.38  g ) and the Moon (0.17  g ). They germinated seeds of thale cress ( Arabidopsis thaliana ) in these devices, and showed in 4-day old seedlings that the balance between cell proliferation and cell growth in the roots was out of whack in both simulated microgravity and Moon gravity levels, but not in a simulated Mars scenario.