Molecular medicine reports, 2018-04, Vol.17 (4), p.5652-5657
2018
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- Autor(en) / Beteiligte
- Titel
- Anticancer activity of caffeic acid n‑butyl ester against A431 skin carcinoma cell line occurs via induction of apoptosis and inhibition of the mTOR/PI3K/AKT signaling pathway
- Ist Teil von
- Molecular medicine reports, 2018-04, Vol.17 (4), p.5652-5657
- Ort / Verlag
- Greece: Spandidos Publications
- Erscheinungsjahr
- 2018
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Skin cancer is one of the primary causes of mortality worldwide. With an increasing frequency of skin cancers, there is an urgent requirement for the development of numerous treatment options. The present study investigated the anticancer activity of caffeic acid n‑butyl ester (CAE) against the A431 skin cancer cell line. Antiproliferative effects were investigated using an MMT assay. Apoptosis was examined by DAPI and Annexin V/fluorescein isothiocyanate and propidium iodide staining. Reactive oxygen species (ROS), mitochondrial membrane potential (MMP) and cell cycle analyses were performed via flow cytometry. Protein expression was determined by western blotting. The findings of the present study demonstrated that among a variety of cancer cell lines, CAE exhibited significant anticancer activity against the A431 skin cancer cell line with a half‑maximal inhibitory concentration of 20 µM. CAE was associated with apoptosis and cell cycle arrest of A431 cells, and induced ROS‑mediated alterations in MMP. In addition, CAE considerably suppressed the expression of some of the important proteins of the phosphoinositide 3‑kinase (PI3K)/protein kinase B (AKT)/mechanistic target of rapamycin (mTOR) cascade. The results of the present study indicated that CAE exerted anticancer effects on the A431 skin carcinoma cell line via the induction of apoptosis and suppression of the PI3K/AKT/mTOR signaling pathway. Therefore, CAE may be beneficial for the development of chemotherapy for skin cancers.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1791-2997eISSN: 1791-3004DOI: 10.3892/mmr.2018.8599Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5866006
- Format
- –
- Schlagworte
- 1-Phosphatidylinositol 3-kinase, AKT protein, Annexin V, Antibiotics, Antineoplastic Agents, Phytogenic - chemistry, Antineoplastic Agents, Phytogenic - pharmacology, Antitumor activity, Apoptosis, Apoptosis - drug effects, Butyl acetate, Caffeic acid, Caffeic Acids - chemistry, Caffeic Acids - pharmacology, Caffeine, Cancer therapies, Care and treatment, Cell cycle, Cell Cycle Checkpoints - drug effects, Cell Line, Tumor, Cell Proliferation - drug effects, Cell Survival - drug effects, Chemical properties, Chemotherapy, Drug dosages, Fibroblasts, Flavonoids, Flow cytometry, Fluorescein isothiocyanate, Genetic aspects, Humans, Inhibitory Concentration 50, Kinases, Lung cancer, Matrix Metalloproteinases - metabolism, Medical research, Membrane potential, Mitochondria, Mortality, Phosphatidylinositol 3-Kinases - metabolism, Physiological aspects, Propidium iodide, Prostate, Protein expression, Proteins, Proto-Oncogene Proteins c-akt - metabolism, Rapamycin, Reactive Oxygen Species, Risk factors, Signal transduction, Signal Transduction - drug effects, Skin cancer, Skin carcinoma, Skin Neoplasms - metabolism, Studies, TOR protein, TOR Serine-Threonine Kinases - metabolism, Tumor cell lines, Western blotting