Details

Autor(en) / Beteiligte

• Le, Ngoc D. B
• Yesilbag Tonga, Gulen
• Mout, Rubul
• Kim, Sung-Tae
• Wille, Marcos E
• Rana, Subinoy
• Dunphy, Karen A
• Jerry, D. Joseph
• Yazdani, Mahdieh
• Ramanathan, Rajesh
• Rotello, Caren M
• Rotello, Vincent M

Titel

Cancer Cell Discrimination Using Host–Guest “Doubled” Arrays

Ist Teil von

• Journal of the American Chemical Society, 2017-06, Vol.139 (23), p.8008-8012

Ort / Verlag

United States: American Chemical Society

Erscheinungsjahr

2017

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• We report a nanosensor that uses cell lysates to rapidly profile the tumorigenicity of cancer cells. This sensing platform uses host–guest interactions between cucurbit[7]­uril and the cationic headgroup of a gold nanoparticle to non-covalently modify the binding of three fluorescent proteins of a multi-channel sensor in situ. This approach doubles the number of output channels to six, providing single-well identification of cell lysates with 100% accuracy. Significantly, this classification could be extended beyond the training set, determining the invasiveness of novel cell lines. The unique fingerprint of these cell lysates required minimal sample quantity (200 ng, ∼1000 cells), making the methodology compatible with microbiopsy technology.