Scientific reports, 2018-01, Vol.8 (1), p.389-389, Article 389
2018
Details
- Autor(en) / Beteiligte
- Titel
- RNA-Seq analysis and comparison of corneal epithelium in keratoconus and myopia patients
- Ist Teil von
- Scientific reports, 2018-01, Vol.8 (1), p.389-389, Article 389
- Ort / Verlag
- England: Nature Publishing Group
- Erscheinungsjahr
- 2018
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Keratoconus is a common degenerative corneal disease that can lead to significant visual morbidity, and both genetic and environmental factors have been implicated in its pathogenesis. We compared the transcriptome of keratoconus and control epithelium using RNA-Seq. Epithelial tissues were obtained prior to surgery from keratoconus and myopia control patients, undergoing collagen cross-linking and photorefractive keratectomy, respectively. We identified major differences in keratoconus linked to cell-cell communication, cell signalling and cellular metabolism. The genes associated with the Hedgehog, Wnt and Notch1 signaling pathways were down-regulated in keratoconus. We also identified plasmolipin and Notch1 as being significantly reduced in keratoconus for both gene and protein expression (p < 0.05). Plasmolipin is a novel protein identified in human corneal epithelium, and has been demonstrated to have a key role in epithelial cell differentiation in other tissues. This study shows altered gene and protein expression of these three proteins in keratoconus, and further studies are clearly warranted to confirm the functional role of these proteins in the pathogenesis of keratoconus.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2045-2322eISSN: 2045-2322DOI: 10.1038/s41598-017-18480-xTitel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5762683
- Format
- –
- Schlagworte
- Adolescent, Adult, Cell differentiation, Cell interactions, Cell signaling, Collagen, Cornea, Cross-linking, Environmental factors, Epithelial cells, Epithelium, Epithelium, Corneal - metabolism, Eye diseases, Female, Gene expression, Gene Expression Profiling - methods, Gene Expression Regulation, Gene Regulatory Networks, Humans, Keratoconus, Keratoconus - genetics, Keratoconus - metabolism, Male, Middle Aged, Morbidity, Myelin and Lymphocyte-Associated Proteolipid Proteins - genetics, Myelin and Lymphocyte-Associated Proteolipid Proteins - metabolism, Myopia, Myopia - genetics, Myopia - metabolism, Pathogenesis, Patients, Protein expression, Proteins, Receptor, Notch1 - genetics, Receptor, Notch1 - metabolism, Ribonucleic acid, RNA, Sequence Analysis, RNA - methods, Signal transduction, Surgery, Transcriptomes, Wnt protein, Wnt Signaling Pathway, Young Adult