Details

Autor(en) / Beteiligte

• Davis, Shaun M
• Thomas, Amanda L
• Liu, Lingzhi
• Campbell, Ian M
• Dierick, Herman A

Titel

Isolation of Aggressive Behavior Mutants in Drosophila Using a Screen for Wing Damage

Ist Teil von

• Genetics (Austin), 2018-01, Vol.208 (1), p.273-282

Ort / Verlag

United States: Genetics Society of America

Erscheinungsjahr

2018

Link zum Volltext

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• Aggression is a complex social behavior that is widespread in nature. To date, only a limited number of genes that affect aggression have been identified, in large part because the complexity of the phenotype makes screening difficult and time-consuming regardless of the species that is studied. We discovered that aggressive group-housed males inflict damage on each other's wings, and show that wing damage negatively affects their ability to fly and mate. Using this wing-damage phenotype, we screened males from ∼1400 chemically mutagenized strains and found ∼40 mutant strains with substantial wing damage. Five of these mutants also had increased aggressive behavior. To identify the causal mutation in one of our top aggressive strains, we used whole-genome sequencing and genomic duplication rescue strategies. We identified a novel mutation in the voltage-gated potassium channel ( ) and show that a nearby previously identified mutation also results in increased aggression. This simple screen can be used to dissect the molecular mechanisms underlying aggression.