Journal of clinical microbiology, 2018-01, Vol.56 (1)
2018
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Details
- Autor(en) / Beteiligte
- Titel
- Rapid Detection of Carbapenemase Production in Enterobacteriaceae by Use of a Modified Paper Strip Carba NP Method
- Ist Teil von
- Journal of clinical microbiology, 2018-01, Vol.56 (1)
- Ort / Verlag
- United States: American Society for Microbiology
- Erscheinungsjahr
- 2018
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- Rapid and accurate detection of carbapenemase-producing (CPE) is important for preventing their spread in health care settings. We compared the performance of the Carba NP (CNP) test using the CLSI tube method with that using a modified paper strip method for the detection of carbapenemases in 390 isolates. The isolates were identified by Hong Kong's carbapenem-resistant surveillance program in 2016 and comprised 213 CPE and 177 carbapenemase-negative isolates. Molecular genotype was used as the reference. The test results were read at different time points for the CLSI method (1 min, 5 min, 1 h, and 2 h) and strip method (1 min and 5 min). The strip CNP and CLSI CNP tests correctly detect carbapenemase production in 93% and 93% of KPC producers, 100% and 38% of IMI producers, 94% and 85% of IMP producers, 98% and 90% of NDM producers, and 29% and 12% of OXA producers, respectively. Overall, the strip method has superior sensitivity to the CLSI method (86% versus 75%, respectively; < 0.001, McNemar test). The specificity of both methods was 100%. By the CLSI method, 27%, 14%, 29%, and 6% of the CPE isolates were positive at 1 min, 5 min, 1 h, and 2 h, respectively. In contrast, by the strip method, 76% of the CPE isolates were positive at 1 min, and an additional 10% were positive at 5 min. In conclusion, the Carba NP test by use of the modified strip method has a higher sensitivity and a shorter assay time than that those by use of the CLSI tube method.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0095-1137eISSN: 1098-660XDOI: 10.1128/jcm.01110-17Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5744197
- Format
- –
- Schlagworte
- Anti-Bacterial Agents - metabolism, Anti-Bacterial Agents - pharmacology, Bacterial Proteins - analysis, Bacterial Proteins - metabolism, Bacteriological Techniques - methods, Bacteriological Techniques - standards, Bacteriology, beta-Lactamases - analysis, beta-Lactamases - metabolism, Carbapenem-Resistant Enterobacteriaceae - drug effects, Carbapenem-Resistant Enterobacteriaceae - enzymology, Carbapenems - metabolism, Carbapenems - pharmacology, Enterobacteriaceae - drug effects, Enterobacteriaceae - enzymology, Hong Kong, Humans, Microbial Sensitivity Tests - standards, Sensitivity and Specificity, Time Factors