Scientific reports, 2017-11, Vol.7 (1), p.15929-10, Article 15929
2017
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- Autor(en) / Beteiligte
- Titel
- MiR-30e inhibits tumor growth and chemoresistance via targeting IRS1 in Breast Cancer
- Ist Teil von
- Scientific reports, 2017-11, Vol.7 (1), p.15929-10, Article 15929
- Ort / Verlag
- London: Nature Publishing Group UK
- Erscheinungsjahr
- 2017
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- MicroRNA-30e (miR-30e) is downregulated in various tumor types. However, its mechanism in inhibiting tumor growth of breast cancer remains to be elucidated. In this study, we found that miR-30e was significantly downregulated in tumor tissues of breast cancer (BC) patients and cell lines, and overexpression of miR-30e inhibited cell proliferation, migration and invasion. To understand the potential mechanism of miR-30e in inhibiting tumor growth, we showed that miR-30e blocked the activation of AKT and ERK1/2 pathways, and the expression of HIF-1α and VEGF via directly targeting IRS1. Moreover, miR-30e regulates cell proliferation, migration, invasion and increases chemosensitivity of MDA-MB-231 cells to paclitaxel by inhibiting its target IRS1. MiR-30e also inhibited tumor growth and suppressed expression of IRS1, AKT, ERK1/2 and HIF-1α in mouse xenograft tumors. To test the clinical relevance of these results, we used 40 pairs of BC tissues and adjacent normal tissues, analyzed the levels of miR-30e and IRS1 expression in these tissues, and found that miR-30e levels were significantly inversely correlated with IRS1 levels in these BC tissues, suggesting the important implication of our findings in translational application for BC diagnostics and treatment in the future.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2045-2322eISSN: 2045-2322DOI: 10.1038/s41598-017-16175-xTitel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5698445
- Format
- –
- Schlagworte
- 13/1, 13/2, 13/31, 13/95, 14/63, 45/22, 631/80/84/2336, 64/60, 692/53/2421, AKT protein, Animals, Base Sequence, Breast cancer, Breast Neoplasms - drug therapy, Breast Neoplasms - genetics, Breast Neoplasms - pathology, Cell growth, Cell Line, Tumor, Cell Movement - drug effects, Cell Movement - genetics, Cell proliferation, Cell Proliferation - drug effects, Cell Proliferation - genetics, Chemoresistance, Down-Regulation - drug effects, Down-Regulation - genetics, Drug Resistance, Neoplasm - drug effects, Drug Resistance, Neoplasm - genetics, Extracellular Signal-Regulated MAP Kinases - metabolism, Female, Gene Expression Regulation, Neoplastic - drug effects, HEK293 Cells, Humanities and Social Sciences, Humans, Insulin Receptor Substrate Proteins - metabolism, Mice, Inbred BALB C, Mice, Nude, MicroRNAs - genetics, MicroRNAs - metabolism, miRNA, multidisciplinary, Neoplasm Invasiveness, Paclitaxel, Paclitaxel - pharmacology, Paclitaxel - therapeutic use, Proto-Oncogene Proteins c-akt - metabolism, Science, Science (multidisciplinary), Signal Transduction - drug effects, Tissues, Tumor cell lines, Tumors, Vascular endothelial growth factor, Xenografts