Details

Autor(en) / Beteiligte

• Dixit, Deobrat
• Wallace, Lisa C
• Lee, Christine
• Miller, Tyler E
• Prager, Briana
• Rich, Jeremy

Titel

STEM-12. TARGETING GLIOMA STEM CELL MIRNA NETWORKS

Ist Teil von

• Neuro-oncology (Charlottesville, Va.), 2017-11, Vol.19 (suppl_6), p.vi228-vi228

Ort / Verlag

US: Oxford University Press

Erscheinungsjahr

2017

Quelle

Oxford Journals 2020 Medicine

Beschreibungen/Notizen

• Glioma stem cells (GSCs) are self-renewing, highly tumorigenic cells that often display therapeutic resistance, supporting a need for discovery of novel molecular targeting strategies. Using a whole genome miRNA screen, we identified novel miRNAs that regulate GSC stemness and survival. Leveraging three patient-derived GSC models, we performed a primary miRNA screen using 2051 miRNA-mimics. The top 5% miRNAs (18 miRNAs) that increased or decreased cell viability were selected for further validation, with 10 passing validation in a secondary screen in six patient-derived GSCs. In vitro limiting dilution assays confirmed that these miRNAs significantly modulate stemness of GSCs. IPA analysis predicted these miRNAs as regulators in key biological processes important for cancer, cell cycle, cell death and survival. Ongoing experiments are dissecting the molecular mechanisms regulated by selected miRNAs and optimizing in vivo targeting. Collectively, this novel miRNA screening may inform novel glioblastoma therapies and provide global gene regulatory networks integral to GSC biology.