Details

Autor(en) / Beteiligte

• Matamoros, Sébastien
• van Hattem, Jarne M
• Arcilla, Maris S
• Willemse, Niels
• Melles, Damian C
• Penders, John
• Vinh, Trung Nguyen
• Thi Hoa, Ngo
• Bootsma, Martin C J
• van Genderen, Perry J
• Goorhuis, Abraham
• Grobusch, Martin
• Molhoek, Nicky
• Oude Lashof, Astrid M L
• Stobberingh, Ellen E
• Verbrugh, Henri A
• de Jong, Menno D
• Schultsz, Constance

Titel

Global phylogenetic analysis of Escherichia coli and plasmids carrying the mcr-1 gene indicates bacterial diversity but plasmid restriction

Ist Teil von

• Scientific reports, 2017-11, Vol.7 (1), p.15364, Article 15364

Ort / Verlag

England: Nature Publishing Group

Erscheinungsjahr

2017

Link zum Volltext

Quelle

EZB-FREE-00999 freely available EZB journals

Beschreibungen/Notizen

• To understand the dynamics behind the worldwide spread of the mcr-1 gene, we determined the population structure of Escherichia coli and of mobile genetic elements (MGEs) carrying the mcr-1 gene. After a systematic review of the literature we included 65 E. coli whole genome sequences (WGS), adding 6 recently sequenced travel related isolates, and 312 MLST profiles. We included 219 MGEs described in 7 Enterobacteriaceae species isolated from human, animal and environmental samples. Despite a high overall diversity, 2 lineages were observed in the E. coli population that may function as reservoirs of the mcr-1 gene, the largest of which was linked to ST10, a sequence type known for its ubiquity in human faecal samples and in food samples. No genotypic clustering by geographical origin or isolation source was observed. Amongst a total of 13 plasmid incompatibility types, the IncI2, IncX4 and IncHI2 plasmids accounted for more than 90% of MGEs carrying the mcr-1 gene. We observed significant geographical clustering with regional spread of IncHI2 plasmids in Europe and IncI2 in Asia. These findings point towards promiscuous spread of the mcr-1 gene by efficient horizontal gene transfer dominated by a limited number of plasmid incompatibility types.