Details

Autor(en) / Beteiligte

• Mercy, Guillaume
• Mozziconacci, Julien
• Scolari, Vittore F.
• Yang, Kun
• Zhao, Guanghou
• Thierry, Agnès
• Luo, Yisha
• Mitchell, Leslie A.
• Shen, Michael
• Shen, Yue
• Walker, Roy
• Zhang, Weimin
• Wu, Yi
• Xie, Ze-xiong
• Luo, Zhouqing
• Cai, Yizhi
• Dai, Junbiao
• Yang, Huanming
• Yuan, Ying-Jin
• Boeke, Jef D.
• Bader, Joel S.
• Muller, Héloïse
• Koszul, Romain

Titel

3D organization of synthetic and scrambled chromosomes

Ist Teil von

• Science (American Association for the Advancement of Science), 2017-03, Vol.355 (6329), p.1050-1050

Ort / Verlag

United States: American Association for the Advancement of Science

Erscheinungsjahr

2017

Quelle

MEDLINE

Beschreibungen/Notizen

• Although the design of the synthetic yeast genome Sc2.0 is highly conservative with respect to gene content, the deletion of several classes of repeated sequences and the introduction of thousands of designer changes may affect genome organization and potentially alter cellular functions. We report here the Hi-C-determined three-dimensional (3D) conformations of Sc2.0 chromosomes. The absence of repeats leads to a smoother contact pattern and more precisely tractable chromosome conformations, and the large-scale genomic organization is globally unaffected by the presence of synthetic chromosome(s). Two exceptions are synIII, which lacks the silent mating-type cassettes, and synXII, specifically when the ribosomal DNA is moved to another chromosome. We also exploit the contact maps to detect rearrangements induced in SCRaMbLE (synthetic chromosome rearrangement and modification by -mediated evolution) strains.