Details

Autor(en) / Beteiligte

• Gao, Lei
• Yang, Zhufeng
• Hiremath, Chitkale
• Zimmerman, Susan E
• Long, Blake
• Brakeman, Paul R
• Mostov, Keith E
• Bryant, David M
• Luby-Phelps, Katherine
• Marciano, Denise K

Titel

Afadin orients cell division to position the tubule lumen in developing renal tubules

Ist Teil von

• Development (Cambridge), 2017-10, Vol.144 (19), p.3511-3520

Ort / Verlag

England: The Company of Biologists Ltd

Erscheinungsjahr

2017

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• In many types of tubules, continuity of the lumen is paramount to tubular function, yet how tubules generate lumen continuity is not known. We recently found that the F-actin-binding protein afadin is required for lumen continuity in developing renal tubules, though its mechanism of action remains unknown. Here, we demonstrate that afadin is required for lumen continuity by orienting the mitotic spindle during cell division. Using an 3D cyst model, we find that afadin localizes to the cell cortex adjacent to the spindle poles and orients the mitotic spindle. In tubules, cell division may be oriented relative to two axes: longitudinal and apical-basal. Unexpectedly, examination of early-stage developing nephron tubules reveals that cell division is not oriented in the longitudinal (or planar-polarized) axis. However, cell division is oriented perpendicular to the apical-basal axis. Absence of afadin leads to misorientation of apical-basal cell division in nephron tubules. Together, these results support a model whereby afadin determines lumen placement by directing apical-basal spindle orientation, resulting in a continuous lumen and normal tubule morphogenesis.