Details

Autor(en) / Beteiligte

• Ichikawa, Takafumi
• Kita, Masahiro
• Matsui, Tsubasa S
• Nagasato, Ayaka Ichikawa
• Araki, Tomohiko
• Chiang, Shian-Huey
• Sezaki, Takuhito
• Kimura, Yasuhisa
• Ueda, Kazumitsu
• Deguchi, Shinji
• Saltiel, Alan R
• Kioka, Noriyuki

Titel

Vinexin family (SORBS) proteins play different roles in stiffness-sensing and contractile force generation

Ist Teil von

• Journal of cell science, 2017-10, Vol.130 (20), p.3517-3531

Ort / Verlag

England: The Company of Biologists Ltd

Erscheinungsjahr

2017

Quelle

MEDLINE

Beschreibungen/Notizen

• Vinexin, c-Cbl associated protein (CAP) and Arg-binding protein 2 (ArgBP2) constitute an adaptor protein family called the vinexin (SORBS) family that is targeted to focal adhesions (FAs). Although numerous studies have focused on each of the SORBS proteins and partially elucidated their involvement in mechanotransduction, a comparative analysis of their function has not been well addressed. Here, we established mouse embryonic fibroblasts that individually expressed SORBS proteins and analysed their functions in an identical cell context. Both vinexin-α and CAP co-localized with vinculin at FAs and promoted the appearance of vinculin-rich FAs, whereas ArgBP2 co-localized with α-actinin at the proximal end of FAs and punctate structures on actin stress fibers (SFs), and induced paxillin-rich FAs. Furthermore, both vinexin-α and CAP contributed to extracellular matrix stiffness-dependent vinculin behaviors, while ArgBP2 stabilized α-actinin on SFs and enhanced intracellular contractile forces. These results demonstrate the differential roles of SORBS proteins in mechanotransduction.