Bio-protocol, 2017-05, Vol.7 (10)
2017
Details
- Autor(en) / Beteiligte
- Titel
- Spore Preparation Protocol for Enrichment of Clostridia from Murine Intestine
- Ist Teil von
- Bio-protocol, 2017-05, Vol.7 (10)
- Ort / Verlag
- United States: Bio-Protocol
- Erscheinungsjahr
- 2017
- Link zum Volltext
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- In recent years, many spore-forming commensal Clostridia found in the gut have been discovered to promote host physiology, immune development, and protection against infections. We provide a detailed protocol for rapid enrichment of spore-forming bacteria from murine intestine. Briefly, contents from the intestinal cecum are collected aerobically, diluted and finally treated with chloroform to enrich for Clostridia spores.