Details

Autor(en) / Beteiligte

• Molina-Moya, Barbara
• Gomgnimbou, Michel Kiréopori
• Lafoz, Carmen
• Lacoma, Alicia
• Prat, Cristina
• Refrégier, Guislaine
• Samper, Sofia
• Dominguez, Jose
• Sola, Christophe

Titel

Molecular Characterization of Mycobacterium tuberculosis Strains with TB-SPRINT

Ist Teil von

• The American journal of tropical medicine and hygiene, 2017-01, Vol.97 (3), p.806-809

Ort / Verlag

United States: American Society of Tropical Medicine and Hygiene

Erscheinungsjahr

2017

Quelle

MEDLINE

Beschreibungen/Notizen

• We evaluated Tuberculosis-Spoligo-Rifampicin-Isoniazid Typing (TB-SPRINT), a microbead-based method for spoligotyping and detection of rifampicin and isoniazid resistance in . For that, 67 complex strains were retrospectively selected. Membrane-based spoligotyping, restriction fragment length polymorphism, DNA sequencing/pyrosequencing of , , and promoter, TB-SPRINT, and SNP typing were performed. Concordance between spoligotyping methods was 99.6% (2,785/2,795 spoligotype data points). For most of the discordant cases, the same lineage was assigned with both methods. Concordance between phenotypic drug susceptibility testing and TB-SPRINT for detecting rifampicin and isoniazid resistance was 98.4% (63/64) and 93.8% (60/64), respectively. Concordance between DNA sequencing/pyrosequencing and TB-SPRINT for detecting mutations in , , and were 98.4% (60/61), 100% (64/64), and 96.9% (62/64), respectively. In conclusion, TB-SPRINT is a rapid and easy-to-perform assay for genotyping and detecting drug resistance in a single tube; therefore, it may be a useful tool to improve epidemiological surveillance.