Details

Autor(en) / Beteiligte

• Borkowski, Leszek
• Sroka-Bartnicka, Anna
• Polkowska, Izabela
• Pawlowska, Marta
• Palka, Krzysztof
• Zieba, Emil
• Slosarczyk, Anna
• Jozwiak, Krzysztof
• Ginalska, Grazyna

Titel

New approach in evaluation of ceramic-polymer composite bioactivity and biocompatibility

Ist Teil von

• Analytical and bioanalytical chemistry, 2017-09, Vol.409 (24), p.5747-5755

Ort / Verlag

Berlin/Heidelberg: Springer Berlin Heidelberg

Erscheinungsjahr

2017

Link zum Volltext

Quelle

SpringerLink (Online service)

Beschreibungen/Notizen

• Regeneration of bone defects was promoted by a novel β-glucan/carbonate hydroxyapatite composite and characterized by Raman spectroscopy, microCT and electron microscopy. The elastic biomaterial with an apatite-forming ability was developed for bone tissue engineering and implanted into the critical-size defects of rabbits’ tibiae. The bone repair process was analyzed on non-decalcified bone/implant sections during a 6-month regeneration period. Using spectroscopic methods, we were able to determine the presence of amides, lipids and assign the areas of newly formed bone tissue. Raman spectroscopy was also used to assess the chemical changes in the composite before and after the implantation process. SEM analyses showed the mineralization degree in the defect area and that the gap size decreased significantly. Microscopic images revealed that the implant debris were interconnected to the poorly mineralized inner side of a new bone tissue. Our study demonstrated that the composite may serve as a biocompatible background for collagen ingrowth and exhibits the advantages of applying Raman spectroscopy, SEM and microCT in studying these samples.