Neuron (Cambridge, Mass.), 2017-08, Vol.95 (4), p.852-868.e8
2017
Details
- Autor(en) / Beteiligte
- Titel
- RNA Docking and Local Translation Regulate Site-Specific Axon Remodeling In Vivo
- Ist Teil von
- Neuron (Cambridge, Mass.), 2017-08, Vol.95 (4), p.852-868.e8
- Ort / Verlag
- United States: Elsevier Inc
- Erscheinungsjahr
- 2017
- Link zum Volltext
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- Nascent proteins can be positioned rapidly at precise subcellular locations by local protein synthesis (LPS) to facilitate localized growth responses. Axon arbor architecture, a major determinant of synaptic connectivity, is shaped by localized growth responses, but it is unknown whether LPS influences these responses in vivo. Using high-resolution live imaging, we examined the spatiotemporal dynamics of RNA and LPS in retinal axons during arborization in vivo. Endogenous RNA tracking reveals that RNA granules dock at sites of branch emergence and invade stabilized branches. Live translation reporter analysis reveals that de novo β-actin hotspots colocalize with docked RNA granules at the bases and tips of new branches. Inhibition of axonal β-actin mRNA translation disrupts arbor dynamics primarily by reducing new branch emergence and leads to impoverished terminal arbors. The results demonstrate a requirement for LPS in building arbor complexity and suggest a key role for pre-synaptic LPS in assembling neural circuits. •Tracking endogenous RNA shows that RNA docking predicts axon branch emergence in vivo•Axon arbor complexity in vivo depends on local protein synthesis•Axonal β-actin synthesis regulates branching by increased branch initiation•Live imaging reveals de novo synthesis of β-actin hotspots during branch formation Wong et al. track endogenous RNA granules in single axons in vivo and show that they predict positions of axonal branch formation in the tectum. They also show that perturbation of local protein synthesis in these axons autonomously regulates branch formation.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0896-6273eISSN: 1097-4199DOI: 10.1016/j.neuron.2017.07.016Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5563073
- Format
- –
- Schlagworte
- Actin, Actins - genetics, Actins - metabolism, Animals, Anisomycin - pharmacology, axon branching, axon guidance, Axons, Axons - physiology, Biotin - metabolism, Blastomeres, Brain-derived neurotrophic factor, Carbocyanines - metabolism, Circuits, Cycloheximide - pharmacology, Deoxyuracil Nucleotides - metabolism, Docking, Embryo, Nonmammalian, Emergence, FRAP, Gene Expression Regulation, Developmental - drug effects, Gene Expression Regulation, Developmental - genetics, Granular materials, High resolution, Image resolution, In Vitro Techniques, Lipopolysaccharides, local protein synthesis, Luminescent Proteins - genetics, Luminescent Proteins - metabolism, mitochondria, Mitochondria - metabolism, Mitochondrial DNA, Morpholinos - pharmacology, Motility, mRNA, Neural networks, neural wiring, Neurons, Oligonucleotides, Antisense - pharmacology, Organ Culture Techniques, Protein biosynthesis, Protein synthesis, Protein Synthesis Inhibitors - pharmacology, Proteins, Retina, Retina - cytology, retinotectal projection, RNA - genetics, RNA - metabolism, RNA labeling, RNA localization, RNA trafficking, Studies, Translation, Xenopus laevis, β-actin