Details

Autor(en) / Beteiligte

• Liu, Jian-Sheng
• Li, Jing-Bo
• Gong, Xin-Wei
• Gong, Hai-Qing
• Zhang, Pu-Ming
• Liang, Pei-Ji
• Lu, Qin-Chi

Titel

Spatiotemporal dynamics of high-K+-induced epileptiform discharges in hippocampal slice and the effects of valproate

Ist Teil von

• Neuroscience bulletin, 2013-02, Vol.29 (1), p.28-36

Ort / Verlag

Heidelberg: Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences

Erscheinungsjahr

2013

Link zum Volltext

Quelle

SpringerLink Journals

Beschreibungen/Notizen

• The epileptic seizure is a dynamic process involving a rapid transition from normal activity to a state of hypersynchronous neuronal discharges. Here we investigated the network properties of epileptiform discharges in hippocampal slices in the presence of high K + concentration (8.5 mmol/L) in the bath, and the effects of the anti-epileptic drug valproate (VPA) on epileptiform discharges, using a microelectrode array. We demonstrated that epileptiform discharges were predominantly initiated from the stratum pyramidale layer of CA3a-b and propagated bi-directionally to CA1 and CA3c. Disconnection of CA3 from CA1 abolished the discharges in CA1 without disrupting the initiation of discharges in CA3. Further pharmacological experiments showed that VPA at a clinically relevant concentration (100 μmol/L) suppressed the propagation speed but not the rate or duration of high-K + -induced discharges. Our findings suggest that pacemakers exist in the CA3a-b region for the generation of epileptiform discharges in the hippocampus. VPA reduces the conduction of such discharges in the network by reducing the propagation speed.