Details

Autor(en) / Beteiligte

• Van Roey, Griet A., PhD
• Vanison, Christopher C., MD
• Wu, Jeffanie
• Huang, Julia H., MSc
• Suh, Lydia A., BSc
• Carter, Roderick G., BSc
• Norton, James E., MSc
• Shintani-Smith, Stephanie, MD
• Conley, David B., MD
• Welch, Kevin C., MD
• Peters, Anju T., MD
• Grammer, Leslie C., MD
• Harris, Kathleen E., BSc
• Hulse, Kathryn E., PhD
• Kato, Atsushi, PhD
• Stevens, Whitney W., MD, PhD
• Kern, Robert C., MD
• Schleimer, Robert P., PhD
• Tan, Bruce K., MD, MSc

Titel

Classical complement pathway activation in the nasal tissue of patients with chronic rhinosinusitis

Ist Teil von

• Journal of allergy and clinical immunology, 2017-07, Vol.140 (1), p.89-100.e2

Ort / Verlag

United States: Elsevier Limited

Erscheinungsjahr

2017

Quelle

Access via ScienceDirect (Elsevier)

Beschreibungen/Notizen

• Background Complement plays a major role in inflammatory diseases, but its involvement and mechanisms of activation in patients with chronic rhinosinusitis (CRS) are not known. Objectives After earlier studies discovering autoantibodies in patients with CRS, we sought to investigate the nature, extent, and location of complement activation in nasal tissue of patients with CRS. Specifically, we were interested in whether antibody-mediated activation through the classical pathway was a major mechanism for complement activation in patients with CRS. Methods Nasal tissue was obtained from patients with CRS and control subjects. Tissue homogenates were analyzed for complement activation products (ELISA–C5b-9, C4d, activated C1, and C5a) and major complement-fixing antibodies (Luminex). Tissue sections were stained for C5b-9, C4d, and laminin. Antibodies were purified with protein A/G columns from nasal polyps (NP), matching patient serum, and control serum and assayed for basement membrane binding by means of ELISA. Results C5b-9 levels were significantly increased in NP tissue compared with uncinate tissue (UT) of patients with chronic rhinosinusitis with nasal polyps (CRSwNP) and those with chronic rhinosinusitis without nasal polyps (CRSsNP; P  < .01). Similarly, C4d levels were increased in NPs compared with UT of patients with CRSwNP, patients with CRSsNP, and control subjects ( P  < .05). Activated C1 levels were also increased in NP tissue compared with UT of patients with CRSsNP and control subjects ( P  < .05) and correlated with levels of C5a ( P  < .01), local immunoglobulins (especially IgM, P  < .0001), and anti–double-stranded DNA IgG ( P  < .05). Immunofluorescence showed that C5b-9 and C4d deposition occurred linearly along the epithelial basement membrane. NP tissue extracts had significantly more anti–basement membrane antibodies than sera from patients with CRSwNP and control subjects ( P  < .0001). Conclusion Levels of C5b-9, C4d, and activated C1 were significantly increased locally in NP tissue. C5b-9 and C4d were almost universally deposited linearly along the basement membrane of NP tissue. Furthermore, activated C1 levels were best correlated with local immunoglobulin and C5a levels. Together, these data suggest that the classical pathway plays a major role in complement activation in patients with CRS.