Details

Autor(en) / Beteiligte

• Arumugakani, Gururaj
• Stephenson, Sophie J
• Newton, Darren J
• Rawstron, Andy
• Emery, Paul
• Doody, Gina M
• McGonagle, Dennis
• Tooze, Reuben M

Titel

Early Emergence of CD19-Negative Human Antibody-Secreting Cells at the Plasmablast to Plasma Cell Transition

Ist Teil von

• The Journal of immunology (1950), 2017-06, Vol.198 (12), p.4618-4628

Ort / Verlag

United States: American Association of Immunologists

Erscheinungsjahr

2017

Quelle

MEDLINE

Beschreibungen/Notizen

• Long-lived human plasma cells (PCs) play central roles in immunity and autoimmunity and are enriched among the subpopulation of CD19 human PCs. However, whether human CD19 PCs are necessarily aged cells that have gradually lost CD19 expression is not known. Assessing peripheral blood samples at steady-state and during the acute response to influenza vaccination in healthy donors, we identify the presence of phenotypic CD19 plasmablasts, the proliferative precursor state to mature PCs, and demonstrate by ELISPOT that these are Ab-secreting cells (ASCs). During the acute response to influenza vaccination, CD19 , CD19 , and CD19 ASCs secrete vaccine-specific Abs and show linked repertoires. To address precursor/product relationships, we use in vitro models that mimic T-dependent and T-independent differentiation, finding that the CD19 state can be established at the plasmablast to PC transition, that CD19 PCs increase as a percentage of surviving PCs in vitro, and that CD19 and CD19 PCs can be maintained independently. These data provide proof-of-principle for the view that newly generated ASCs can acquire a mature PC phenotype that is accompanied by loss of CD19 expression at an early stage of differentiation and that aging is not an obligate requirement for a CD19 state to be established.