Details
- Autor(en) / Beteiligte
- Titel
- De novo DNA methylation during monkey pre-implantation embryogenesis
- Ist Teil von
- Cell research, 2017-04, Vol.27 (4), p.526-539
- Ort / Verlag
- England: Nature Publishing Group
- Erscheinungsjahr
- 2017
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Critical epigenetic regulation of primate embryogenesis entails DNA methylome changes. Here we report ge- nome-wide composition, patterning, and stage-specific dynamics of DNA methylation in pre-implantation rhesus monkey embryos as well as male and female gametes studied using an optimized tagmentation-based whole-genome bisulfite sequencing method. We show that upon fertilization, both paternal and maternal genomes undergo active DNA demethylation, and genome-wide de novo DNA methylation is also initiated in the same period. By the 8-cell stage, remethylation becomes more pronounced than demethylation, resulting in an increase in global DNA methyla- tion. Promoters of genes associated with oxidative phosphorylation are preferentially remethylated at the 8-cell stage, suggesting that this mode of energy metabolism may not be favored. Unlike in rodents, X chromosome inactivation is not observed during monkey pre-implantation development. Our study provides the first comprehensive illustration of the ＇wax and wane＇ phases of DNA methylation dynamics. Most importantly, our DNA methyitransferase loss-of- function analysis indicates that DNA methylation influences early monkey embryogenesis.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1001-0602eISSN: 1748-7838DOI: 10.1038/cr.2017.25Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5385613
- Format
- –
- Schlagworte
- Animals, CpG Islands - genetics, DNA (Cytosine-5-)-Methyltransferases - metabolism, DNA Demethylation, DNA Methylation - genetics, DNA Methyltransferase 3A, DNA Methyltransferase 3B, DNA甲基化, Embryo Implantation - genetics, Embryo, Mammalian - metabolism, Female, Gene Knockdown Techniques, Germ Cells - metabolism, Haplorhini, Macaca mulatta, Male, Mice, Original, X Chromosome Inactivation - genetics, 猴, 着床前, 胚胎