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Novel Cadmium Resistance Determinant in Listeria monocytogenes
Ist Teil von
Applied and environmental microbiology, 2017-03, Vol.83 (5), p.E02580
Ort / Verlag
United States: American Society for Microbiology
Erscheinungsjahr
2017
Link zum Volltext
Quelle
MEDLINE
Beschreibungen/Notizen
is a foodborne pathogen that can cause severe disease (listeriosis) in susceptible individuals. It is ubiquitous in the environment and often exhibits resistance to heavy metals. One of the determinants that enables
to tolerate exposure to cadmium is the
efflux system, with CadA being a P-type ATPase. Three different
genes (designated
to
) were previously characterized in
A novel putative cadmium resistance gene (
) was recently identified through whole-genome sequencing, but experimental confirmation for its involvement in cadmium resistance is lacking. In this study, we characterized
in
strain F8027, a cadmium-resistant strain of serotype 4b. By screening a mariner-based transposon library of this strain, we identified a mutant with reduced tolerance to cadmium and that harbored a single transposon insertion in
The tolerance to cadmium was restored by genetic complementation with the cadmium resistance cassette (
), and enhanced cadmium tolerance was conferred to two unrelated cadmium-sensitive strains via heterologous complementation with
Cadmium exposure induced
expression, even at noninhibitory levels. Virulence assessments in the
model suggested that a functional
suppressed virulence, potentially promoting commensal colonization of the insect larvae. Biofilm assays suggested that
inactivation reduced biofilm formation. These data not only confirm
as a novel cadmium resistance determinant in
but also provide evidence for roles in virulence and biofilm formation.
is an intracellular foodborne pathogen causing the disease listeriosis, which is responsible for numerous hospitalizations and deaths every year. Among the adaptations that enable the survival of
in the environment are the abilities to persist in biofilms, grow in the cold, and tolerate toxic compounds, such as heavy metals. Here, we characterized a novel determinant that was recently identified on a larger mobile genetic island through whole-genome sequencing. This gene (
) was found to be responsible for cadmium detoxification and to be a divergent member of the Cad family of cadmium efflux pumps. Virulence assessments in a
model suggested that
may suppress virulence. Additionally,
may be involved in the ability of
to form biofilms. Beyond the role in cadmium detoxification, the involvement of
in other cellular functions potentially explains its retention and wide distribution in
.