Details

Autor(en) / Beteiligte

• Makhijani, Kalpana
• To, Tsz-Leung
• Ruiz-González, Rubén
• Lafaye, Céline
• Royant, Antoine
• Shu, Xiaokun

Titel

Precision Optogenetic Tool for Selective Single- and Multiple-Cell Ablation in a Live Animal Model System

Ist Teil von

• Cell chemical biology, 2017-01, Vol.24 (1), p.110-119

Ort / Verlag

United States: Elsevier Ltd

Erscheinungsjahr

2017

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Cell ablation is a strategy to study cell lineage and function during development. Optogenetic methods are an important cell-ablation approach, and we have previously developed a mini singlet oxygen generator (miniSOG) tool that works in the living Caenorhabditis elegans. Here, we use directed evolution to generate miniSOG2, an improved tool for cell ablation via photogenerated reactive oxygen species. We apply miniSOG2 to a far more complex model animal system, Drosophila melanogaster, and demonstrate that it can be used to kill a single neuron in a Drosophila larva. In addition, miniSOG2 is able to photoablate a small group of cells in one of the larval wing imaginal discs, resulting in an adult with one incomplete and one normal wing. We expect miniSOG2 to be a useful optogenetic tool for precision cell ablation at a desired developmental time point in live animals, thus opening a new window into cell origin, fate and function, tissue regeneration, and developmental biology. [Display omitted] •An efficient photosensitizer miniSOG2 is engineered using directed evolution•miniSOG2 enables precision photoablation of single neurons in live Drosophila•miniSOG2 allows optogenetic ablation of cells in wing imaginal disc A genetically encoded photosensitizer is engineered to efficiently generate reactive oxygen species, which activate caspase and cell apoptosis. It enables precision optogenetic ablation of single neurons in live Drosophila and can be an important tool for studying developmental biology.