Details

Autor(en) / Beteiligte

• Velasco‐Lozano, Susana
• Benítez‐Mateos, Ana I.
• López‐Gallego, Fernando

Titel

Co‐immobilized Phosphorylated Cofactors and Enzymes as Self‐Sufficient Heterogeneous Biocatalysts for Chemical Processes

Ist Teil von

• Angewandte Chemie International Edition, 2017-01, Vol.56 (3), p.771-775

Auflage

International ed. in English

Ort / Verlag

Germany: Wiley Subscription Services, Inc

Erscheinungsjahr

2017

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Enzyme cofactors play a major role in biocatalysis, as many enzymes require them to catalyze highly valuable reactions in organic synthesis. However, the cofactor recycling is often a hurdle to implement enzymes at the industrial level. The fabrication of heterogeneous biocatalysts co‐immobilizing phosphorylated cofactors (PLP, FAD+, and NAD+) and enzymes onto the same solid material is reported to perform chemical reactions without exogeneous addition of cofactors in aqueous media. In these self‐sufficient heterogeneous biocatalysts, the immobilized enzymes are catalytically active and the immobilized cofactors catalytically available and retained into the solid phase for several reaction cycles. Finally, we have applied a NAD+‐dependent heterogeneous biocatalyst to continuous flow asymmetric reduction of prochiral ketones, thus demonstrating the robustness of this approach for large scale biotransformations. Flatmates: The co‐immobilization of enzymes and cofactors on the same porous particles results in a self‐sufficient heterogeneous biocatalyst that performs a chemical reaction without addition of exogenous cofactors. These systems are easily integrated into flow reactors as packed columns for continuous biotransformations.