Details

Autor(en) / Beteiligte

• Guhathakurta, Subhrangshu
• Evangelista, Baggio A
• Ghosh, Susmita
• Basu, Sambuddha
• Kim, Yoon-Seong

Titel

Hypomethylation of intron1 of α-synuclein gene does not correlate with Parkinson's disease

Ist Teil von

• Molecular brain, 2017-02, Vol.10 (1), p.6-6, Article 6

Ort / Verlag

England: BioMed Central

Erscheinungsjahr

2017

Quelle

SpringerLink

Beschreibungen/Notizen

• Deregulation of α-synuclein encoding gene (SNCA) is one of the important facets of Parkinson's disease (PD) research. DNA methylation status of SNCA-intron1 has been shown to regulate the α-synuclein expression. The present study is aimed at investigating whether methylation of SNCA-intron1 is associated with higher expression of α-synuclein in PD. We have investigated the intron1 methylation status from 16 post-mortem brain samples comprised of 8 PD and 8 control subjects using bisulfite sequencing. We further correlated this methylation status with α-synuclein protein levels in substantia nigra of that individual using western blot analysis. We did not observe any significant difference in methylation of SNCA-intron1 region between PD and control samples. Moreover, no correlation was observed between methylation of SNCA-intron1 with α-synuclein level. Methylation of SNCA-intron1 region does not correlate with α-synuclein expression in PD samples.