Details

Autor(en) / Beteiligte

• Osakabe, Akihisa
• Arimura, Yasuhiro
• Matsumoto, Syota
• Horikoshi, Naoki
• Sugasawa, Kaoru
• Kurumizaka, Hitoshi

Titel

Polymorphism of apyrimidinic DNA structures in the nucleosome

Ist Teil von

• Scientific reports, 2017-01, Vol.7 (1), p.41783-41783, Article 41783

Ort / Verlag

England: Nature Publishing Group

Erscheinungsjahr

2017

Quelle

MEDLINE

Beschreibungen/Notizen

• Huge amounts (>10,000/day) of apurinic/apyrimidinic (AP) sites are produced in genomes, but their structures in chromatin remain undetermined. We determined the crystal structure of the nucleosome containing AP-site analogs at two symmetric sites, which revealed structural polymorphism: one forms an inchworm configuration without an empty space at the AP site, and the other forms a B-form-like structure with an empty space and the orphan base. This unexpected inchworm configuration of the AP site is important to understand the AP DNA repair mechanism, because it may not be recognized by the major AP-binding protein, APE1, during the base excision repair process.