Details

Autor(en) / Beteiligte

• Joehanes, Roby
• Zhang, Xiaoling
• Huan, Tianxiao
• Yao, Chen
• Ying, Sai-Xia
• Nguyen, Quang Tri
• Demirkale, Cumhur Yusuf
• Feolo, Michael L
• Sharopova, Nataliya R
• Sturcke, Anne
• Schäffer, Alejandro A
• Heard-Costa, Nancy
• Chen, Han
• Liu, Po-Ching
• Wang, Richard
• Woodhouse, Kimberly A
• Tanriverdi, Kahraman
• Freedman, Jane E
• Raghavachari, Nalini
• Dupuis, Josée
• Johnson, Andrew D
• O'Donnell, Christopher J
• Levy, Daniel
• Munson, Peter J

Titel

Integrated genome-wide analysis of expression quantitative trait loci aids interpretation of genomic association studies

Ist Teil von

• Genome Biology, 2017-01, Vol.18 (1), p.16-16, Article 16

Ort / Verlag

England: BioMed Central

Erscheinungsjahr

2017

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Identification of single nucleotide polymorphisms (SNPs) associated with gene expression levels, known as expression quantitative trait loci (eQTLs), may improve understanding of the functional role of phenotype-associated SNPs in genome-wide association studies (GWAS). The small sample sizes of some previous eQTL studies have limited their statistical power. We conducted an eQTL investigation of microarray-based gene and exon expression levels in whole blood in a cohort of 5257 individuals, exceeding the single cohort size of previous studies by more than a factor of 2. We detected over 19,000 independent lead cis-eQTLs and over 6000 independent lead trans-eQTLs, targeting over 10,000 gene targets (eGenes), with a false discovery rate (FDR) < 5%. Of previously published significant GWAS SNPs, 48% are identified to be significant eQTLs in our study. Some trans-eQTLs point toward novel mechanistic explanations for the association of the SNP with the GWAS-related phenotype. We also identify 59 distinct blocks or clusters of trans-eQTLs, each targeting the expression of sets of six to 229 distinct trans-eGenes. Ten of these sets of target genes are significantly enriched for microRNA targets (FDR < 5%). Many of these clusters are associated in GWAS with multiple phenotypes. These findings provide insights into the molecular regulatory patterns involved in human physiology and pathophysiology. We illustrate the value of our eQTL database in the context of a recent GWAS meta-analysis of coronary artery disease and provide a list of targeted eGenes for 21 of 58 GWAS loci.