Scientific reports, 2017-01, Vol.7 (1), p.41271, Article 41271
2017
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- Autor(en) / Beteiligte
- Titel
- Tryptophan-2,3-Dioxygenase (TDO) deficiency is associated with subclinical neuroprotection in a mouse model of multiple sclerosis
- Ist Teil von
- Scientific reports, 2017-01, Vol.7 (1), p.41271, Article 41271
- Ort / Verlag
- England: Nature Publishing Group
- Erscheinungsjahr
- 2017
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- The catabolism of tryptophan to immunosuppressive and neuroactive kynurenines is a key metabolic pathway regulating immune responses and neurotoxicity. The rate-limiting step is controlled by indoleamine-2,3-dioxygenase (IDO) and tryptophan-2,3-dioxygenase (TDO). IDO is expressed in antigen presenting cells during immune reactions, hepatic TDO regulates blood homeostasis of tryptophan and neuronal TDO influences neurogenesis. While the role of IDO has been described in multiple immunological settings, little is known about TDO's effects on the immune system. TDO-deficiency is neuroprotective in C. elegans and Drosophila by increasing tryptophan and specific kynurenines. Here we have determined the role of TDO in autoimmunity and neurodegeneration in experimental autoimmune encephalomyelitis (EAE), a model of multiple sclerosis. We created reporter-TDO mice for in vivo imaging to show that hepatic but not CNS TDO expression is activated during EAE. TDO deficiency did not influence myelin-specific T cells, leukocyte infiltration into the CNS, demyelination and disease activity. TDO-deficiency protected from neuronal loss in the spinal cord but not in the optic nerves. While this protection did not translate to an improved overt clinical outcome, our data suggest that spatially distinct neuroprotection is conserved in mammals and support TDO as a potential target for treatment of diseases associated with neurodegeneration.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2045-2322eISSN: 2045-2322DOI: 10.1038/srep41271Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5259766
- Format
- –
- Schlagworte
- Animals, Antigen-presenting cells, Autoimmunity, Cell Differentiation, Cell Survival, Central nervous system, Cloning, Molecular, Demyelination, Dioxygenase, Disease Models, Animal, Encephalomyelitis, Autoimmune, Experimental - enzymology, Encephalomyelitis, Autoimmune, Experimental - pathology, Experimental allergic encephalomyelitis, Genes, Reporter, Homeostasis, Immune response, Immune system, Immunosuppression, Inflammation - pathology, Liver - enzymology, Liver - pathology, Lymphocyte Activation, Lymphocytes T, Mice, Inbred C57BL, Mice, Transgenic, Multiple sclerosis, Multiple Sclerosis - enzymology, Multiple Sclerosis - pathology, Multiple Sclerosis - prevention & control, Myelin, Nerves, Neurodegeneration, Neurogenesis, Neuroglia - metabolism, Neuroglia - pathology, Neurons - metabolism, Neurons - pathology, Neuroprotection, Neurotoxicity, Phenotype, Rodents, Spinal cord, T-Lymphocytes - immunology, Tryptophan, Tryptophan Oxygenase - deficiency, Tryptophan Oxygenase - metabolism