Details

Autor(en) / Beteiligte

• Gaglio, Daniela
• Valtorta, Silvia
• Ripamonti, Marilena
• Bonanomi, Marcella
• Damiani, Chiara
• Todde, Sergio
• Negri, Alfredo Simone
• Sanvito, Francesca
• Mastroianni, Fabrizia
• Di Campli, Antonella
• Turacchio, Gabriele
• Di Grigoli, Giuseppe
• Belloli, Sara
• Luini, Alberto
• Gilardi, Maria Carla
• Colangelo, Anna Maria
• Alberghina, Lilia
• Moresco, Rosa Maria

Titel

Divergent in vitro/in vivo responses to drug treatments of highly aggressive NIH-Ras cancer cells: a PET imaging and metabolomics-mass-spectrometry study

Ist Teil von

• Oncotarget, 2016-08, Vol.7 (32), p.52017-52031

Ort / Verlag

United States: Impact Journals LLC

Erscheinungsjahr

2016

Link zum Volltext

Quelle

EZB-FREE-00999 freely available EZB journals

Beschreibungen/Notizen

• Oncogenic K-ras is capable to control tumor growth and progression by rewiring cancer metabolism. In vitro NIH-Ras cells convert glucose to lactate and use glutamine to sustain anabolic processes, but their in vivo environmental adaptation and multiple metabolic pathways activation ability is poorly understood. Here, we show that NIH-Ras cancer cells and tumors are able to coordinate nutrient utilization to support aggressive cell proliferation and survival. Using PET imaging and metabolomics-mass spectrometry, we identified the activation of multiple metabolic pathways such as: glycolysis, autophagy recycling mechanism, glutamine and serine/glycine metabolism, both under physiological and under stress conditions. Finally, differential responses between in vitro and in vivo systems emphasize the advantageous and uncontrolled nature of the in vivo environment, which has a pivotal role in controlling the responses to therapy.