Genes & development, 2016-12, Vol.30 (24), p.2663-2668
2016
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Details
- Autor(en) / Beteiligte
- Titel
- Protein splicing of a recombinase intein induced by ssDNA and DNA damage
- Ist Teil von
- Genes & development, 2016-12, Vol.30 (24), p.2663-2668
- Ort / Verlag
- United States: Cold Spring Harbor Laboratory Press
- Erscheinungsjahr
- 2016
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Inteins (or protein introns) autocatalytically excise themselves through protein splicing. We challenge the long-considered notion that inteins are merely molecular parasites and posit that some inteins evolved to regulate host protein function. Here we show substrate-induced and DNA damage-induced splicing, in which an archaeal recombinase RadA intein splices dramatically faster and more accurately when provided with ssDNA. This unprecedented example of intein splicing stimulation by the substrate of the invaded host protein provides compelling support in favor of inteins acting as pause buttons to arrest protein function until needed; then, an immediate activity switch is triggered, representing a new form of post-translational control.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0890-9369eISSN: 1549-5477DOI: 10.1101/gad.289280.116Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5238726
- Format
- –
- Schlagworte
- Archaeal Proteins - genetics, Archaeal Proteins - metabolism, DNA Damage - genetics, DNA, Single-Stranded - metabolism, DNA-Binding Proteins - genetics, DNA-Binding Proteins - metabolism, Escherichia coli - genetics, Gene Expression Regulation - genetics, Inteins - genetics, Models, Biological, Mutation, Protein Binding, Protein Processing, Post-Translational, Protein Splicing - genetics, Research Communication