Details

Autor(en) / Beteiligte

• Li, Cheng-Gang
• Pu, Meng-Fan
• Li, Chun-Zhu
• Gao, Man
• Liu, Ming-Xia
• Yu, Cun-Zhi
• Yan, Hong
• Peng, Chun
• Zhao, Yang
• Li, Yu
• Ma, Ze-Long
• Qi, Xin-Ming
• Wang, Yi-Zheng
• Miao, Ling-Ling
• Ren, Jin

Titel

MicroRNA-1304 suppresses human non-small cell lung cancer cell growth in vitro by targeting heme oxygenase-1

Ist Teil von

• Acta pharmacologica Sinica, 2017-01, Vol.38 (1), p.110-119

Ort / Verlag

United States: Nature Publishing Group

Erscheinungsjahr

2017

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Previous studies have shown that microRNA-1304 （miR-1304） is dysregulated in certain types of cancers, including non-small cell lung cancer （NSCLC）, and might be involved in tumor survival and/or growth. In this study we investigated the direct target of miR-1304 and its function in NSCLC in vitro. Human lung adenocarcinoma cell lines （A549 and NCI-H1975） were studied. The cell proliferation and survival were investigated via cell counting, MTT and colony-formation assays. Cell apoptosis and cell cycle were examined using annexin V-PE/7-AAD and PI staining assays, respectively. The dual-luciferase reporter assay was used to verify post-transcriptional regulation of heme oxygenase-1 （HO-1） by miR-1304. CRISPR/Cas9 was used to deplete endogenous miR-1304. Overexpression of MiR-1304 significantly decreased the number and viability of NSCLC cells and colony formation, and induced cell apoptosis and Go/ G~ phase cell cycle arrest. HO-1 was demonstrated to be a direct target of miR-1304 in NSCLC cells. Restoration of HO-1 expression by hemin （20 IJmol/L） abolished the inhibition of miR-1304 on cell growth and rescued miR-1304-induced apoptosis in A549 cells. Suppression of endogenous miR-1304 with anti-1304 significantly increased HO-1 expression and promoted cell growth and survival in A549 cells. In 17 human NSCLC tissue samples, miR-1304 expression was significantly decreased, while HO-1 expression was significantly increased as compared to normal lung tissues. MicroRNA-1304 is a tumor suppressor and HO-1 is its direct target in NSCLC. The results suggest the potential for miR-1304 as a therapeutic target for NSCLC.