Acta pharmacologica Sinica, 2017-01, Vol.38 (1), p.90-99
2017
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- 2-Hydroxy-3-methoxybenzoic acid attenuates mast cell-mediated allergic reaction in mice via modulation of the FcεRI signaling pathway
- Ist Teil von
- Acta pharmacologica Sinica, 2017-01, Vol.38 (1), p.90-99
- Ort / Verlag
- United States: Nature Publishing Group
- Erscheinungsjahr
- 2017
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Mast ceils are important effector cells in immunoglobulin （Ig） E-mediated allergic reactions such as asthma, atopic dermatitis and rhinitis. Vanillic acid, a natural product, has shown anti-oxidant and anti-inflammatory activities, in the present study, we investigated the anti-allergic inflammatory effects of ortho-vanillic acid （2-hydroxy-3-methoxybenzoic acid, o-VA） that was a derivative of vanillic acid isolated from Amomum xanthioides, in mouse anaphylaxis models, oral administration of o-VA （2, 10, 50 mg/kg） dose-dependently attenuated ovalbumin-induced active systemic anaphylaxis and IgE-mediated cutaneous allergic reactions such as hypothermia, histamine release, IgE production and vasodilation; administration of o-VA also suppressed the mast cell degranulator compound 48/80-induced anaphylaxis. In cultured mast cell line RBL-2H3 and isolated rat peritoneal mast cells in vitro, pretreatment with o-VA （1-100 pmol/L） dose-dependently inhibited DNP-HSA-induced degranulation of mast cells by decreasing the intracellular free calcium level, and suppressed the expression of pro-inflammatory cytokines TNF-α and IL-4. Pretreatment of RBL-2H3 cells with o-VA suppressed DNP-HSA-induced phosphorylation of Lyn, Syk, Akt, and the nuclear translocation of nuclear factor-κB. In conclusion, o-VA suppresses the mast cell-mediated allergic inflammatory response by blocking the signaling pathways downstream of high affinity IgE receptor （FcsRI） on the surface of mast cells.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1671-4083eISSN: 1745-7254DOI: 10.1038/aps.2016.112Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5220539
- Format
- –
- Schlagworte
- Anaphylaxis - chemically induced, Anaphylaxis - drug therapy, Anaphylaxis - immunology, Animals, Benzoates - pharmacology, Benzoates - therapeutic use, Calcium - metabolism, Cell Degranulation - drug effects, Cells, Cultured, Dinitrophenols - antagonists & inhibitors, Dose-Response Relationship, Drug, Hypersensitivity - drug therapy, Hypersensitivity - immunology, Immunoglobulin E - drug effects, Inflammation Mediators - metabolism, Male, Mast Cells - drug effects, Mast Cells - immunology, Mice, NF-kappa B - metabolism, Original, Ovalbumin - antagonists & inhibitors, p-Methoxy-N-methylphenethylamine - antagonists & inhibitors, Phosphorylation - drug effects, Rats, Receptors, IgE - antagonists & inhibitors, Receptors, IgE - immunology, Serum Albumin - antagonists & inhibitors, Signal Transduction - drug effects, Vanillic Acid - analogs & derivatives, 介导, 信号通路, 免疫球蛋白E, 变态反应, 小鼠, 肥大细胞, 过敏反应