Clinical cancer research, 2017-01, Vol.23 (1), p.204-213
2017
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- Autor(en) / Beteiligte
- Titel
- Identification of Existing Drugs That Effectively Target NTRK1 and ROS1 Rearrangements in Lung Cancer
- Ist Teil von
- Clinical cancer research, 2017-01, Vol.23 (1), p.204-213
- Ort / Verlag
- United States: American Association for Cancer Research Inc
- Erscheinungsjahr
- 2017
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Efforts to discover drugs that overcome resistance to targeted therapies in patients with rare oncogenic alterations, such as NTRK1 and ROS1 rearrangements, are complicated by the cost and protracted timeline of drug discovery. In an effort to identify inhibitors of NTRK1 and ROS1, which are aberrantly activated in some patients with non-small cell lung cancer (NSCLC), we created and screened a library of existing targeted drugs against Ba/F3 cells transformed with these oncogenes. This screen identified the FDA-approved drug cabozantinib as a potent inhibitor of CD74-ROS1-transformed Ba/F3, including the crizotinib-resistant mutants G2032R and L2026M (IC = 9, 26, and 11 nmol/L, respectively). Cabozantinib inhibited CD74-ROS1-transformed Ba/F3 cells more potently than brigatinib (wild-type/G2032R/L2026M IC = 30/170/200 nmol/L, respectively), entrectinib (IC = 6/2,200/3,500 nmol/L), and PF-06463922 (IC = 1/270/2 nmol/L). Cabozantinib inhibited ROS1 autophosphorylation and downstream ERK activation in transformed Ba/F3 cells and in patient-derived tumor cell lines. The IGF-1R inhibitor BMS-536924 potently inhibited CD74-NTRK1-transformed compared with parental Ba/F3 cells (IC = 19 nmol/L vs. > 470 nmol/L). A patient with metastatic ROS1-rearranged NSCLC with progression on crizotinib was treated with cabozantinib and experienced a partial response. While acquired resistance to targeted therapies is challenging, this study highlights that existing agents may be repurposed to overcome drug resistance and identifies cabozantinib as a promising treatment of ROS1-rearranged NSCLC after progression on crizotinib. Clin Cancer Res; 23(1); 204-13. ©2016 AACR.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1078-0432eISSN: 1557-3265DOI: 10.1158/1078-0432.CCR-15-1601Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5203969
- Format
- –
- Schlagworte
- Aberration, Animals, Antineoplastic Agents - chemistry, Antineoplastic Agents - pharmacology, Cancer, Cell Line, Tumor, Drug development, Drug discovery, Drug resistance, Drug Screening Assays, Antitumor, Drugs, Experimental design, Gene Rearrangement, Humans, Inhibitors, Insulin-like growth factors, Lung cancer, Lung Neoplasms - diagnosis, Lung Neoplasms - drug therapy, Lung Neoplasms - genetics, Metastases, Mice, Models, Molecular, Molecular Conformation, Molecular Targeted Therapy, Mutants, Mutation, Non-small cell lung carcinoma, Patients, Phosphorylation, Protein Kinase Inhibitors - chemistry, Protein Kinase Inhibitors - pharmacology, Proto-Oncogene Proteins - antagonists & inhibitors, Proto-Oncogene Proteins - chemistry, Proto-Oncogene Proteins - genetics, Receptor Protein-Tyrosine Kinases - antagonists & inhibitors, Receptor Protein-Tyrosine Kinases - chemistry, Receptor Protein-Tyrosine Kinases - genetics, Receptor, trkA - antagonists & inhibitors, Receptor, trkA - chemistry, Receptor, trkA - genetics, Resistant mutant, Signal Transduction - drug effects, Small Molecule Libraries, Structure-Activity Relationship, Target acquisition, Tomography, X-Ray Computed, Treatment Outcome, Tumor cell lines